不同浓度重组白血病抑制因子对小鼠胚胎体外不同发育阶段的影响
Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro.
作者信息
Tsai H D, Chang C C, Hsieh Y Y, Hsu L W, Chang S C, Lo H Y
机构信息
Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
出版信息
J Assist Reprod Genet. 2000 Jul;17(6):352-5. doi: 10.1023/a:1009413329977.
PURPOSE
To assess the influence of different concentrations of recombinant human leukemia inhibitory factor (LIF) on the in vitro development of mouse embryos.
METHODS
The 2- to 4-cell embryos of CB6F1 mice were cultured in the human tubal fluid (HTF) media containing different concentrations of LIF. Mouse embryos were divided into seven groups: (1) HTF; (2) 1500 IU/ml LIF; (3) 1000 IU/ml LIF; (4) 750 IU/ml LIF; (5) 500 IU/ml LIF; (6) 250 IU/ml LIF; (7) 125 IU/ml LIF. The embryonic numbers of different stages including 5-8 cell, 9-16 cell, morula, blastocyst, and hatching blastocyst were recorded.
RESULTS
The percentage of early embryo stage (2-cell embryos to 6- to 16-cell stages) in all groups were nonsignificantly different. There were higher formation rates of preimplantation embryos (morula to hatching blastocyst) in groups 2, 3, 4, and 5 than in groups 1, 6 and 7.
CONCLUSIONS
LIF has positive effects on preimplantation embryo development and has nonsignificant influence on the early embryo development. The lowest concentration of LIF which could provide the optimal embryo development is 500 IU/ml.
目的
评估不同浓度重组人白血病抑制因子（LIF）对小鼠胚胎体外发育的影响。
方法
将CB6F1小鼠的2至4细胞期胚胎培养于含有不同浓度LIF的人输卵管液（HTF）培养基中。小鼠胚胎分为七组：（1）HTF；（2）1500 IU/ml LIF；（3）1000 IU/ml LIF；（4）750 IU/ml LIF；（5）500 IU/ml LIF；（6）250 IU/ml LIF；（7）125 IU/ml LIF。记录不同阶段（包括5至8细胞、9至16细胞、桑葚胚、囊胚和孵化囊胚）的胚胎数量。
结果
所有组中早期胚胎阶段（2细胞胚胎至6至16细胞阶段）的百分比无显著差异。第2、3、4和5组中着床前胚胎（桑葚胚至孵化囊胚）的形成率高于第1、6和7组。
结论
LIF对着床前胚胎发育有积极作用，对早期胚胎发育影响不显著。能提供最佳胚胎发育的LIF最低浓度为500 IU/ml。
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