大肠杆菌的yiaR和yiaS基因在内源性生成的L-木酮糖代谢中的作用。
Role of the yiaR and yiaS genes of Escherichia coli in metabolism of endogenously formed L-xylulose.
作者信息
Ibañez E, Gimenez R, Pedraza T, Baldoma L, Aguilar J, Badia J
机构信息
Department of Biochemistry, School of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
出版信息
J Bacteriol. 2000 Aug;182(16):4625-7. doi: 10.1128/JB.182.16.4625-4627.2000.
Abstract
Genes yiaP and yiaR of the yiaKLMNOPQRS cluster of Escherichia coli are required for the metabolism of the endogenously formed L-xylulose, whereas yiaS is required for this metabolism only in araD mutants. Like AraD, YiaS was shown to have L-ribulose-5-phosphate 4-epimerase activity. Similarity of YiaR to several 3-epimerases suggested that this protein could catalyze the conversion of L-xylulose-5-phosphate into L-ribulose-5-phosphate, thus completing the pathway between L-xylulose and the general metabolism.
摘要
大肠杆菌yiaKLMNOPQRS基因簇中的yiaP和yiaR基因是内源性生成的L-木酮糖代谢所必需的，而yiaS仅在araD突变体中参与该代谢过程。与AraD一样，YiaS被证明具有L-核糖-5-磷酸4-差向异构酶活性。YiaR与几种3-差向异构酶的相似性表明，该蛋白可以催化L-木酮糖-5-磷酸转化为L-核糖-5-磷酸，从而完成L-木酮糖与一般代谢之间的途径。
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