Nakagami T, Watanabe I, Murakami A, Okisaka S, Ebihara N
Department of Ophthalmology, Hamamatsu University School of Medicine, Shizuoka, Japan.
Jpn J Ophthalmol. 2000 May-Jun;44(3):193-7. doi: 10.1016/s0021-5155(99)00214-2.
To investigate the possible role of stem cell factor (SCF) in the pathogenesis of pterygium.
The localization of SCF was examined immunohistochemically in excised tissue from 4 primary pterygia and 5 normal conjunctival specimens.
Three of the four pterygia showed strong immunoreactivity of SCF in the subepithelial connective tissue at the cap area. This immunoreactivity was completely blocked by using a primary antibody preincubated with recombinant SCF. The SCF-positive cells were identified as a population of fibroblasts by immunostaining for vimentin and prolyl 4-hydroxylase in adjacent sections. No apparent immunoreactivity of SCF was observed in the subepithelial connective tissues in the head and body of the pterygia and in the normal conjunctiva.
Stem cell factor is overexpressed in fibroblasts at the cap area of most pterygia.
探讨干细胞因子（SCF）在翼状胬肉发病机制中的可能作用。
采用免疫组织化学方法检测4例原发性翼状胬肉切除组织及5例正常结膜标本中SCF的定位。
4例翼状胬肉中有3例在头部区域的上皮下结缔组织中显示出SCF的强免疫反应性。通过使用与重组SCF预孵育的一抗，这种免疫反应性被完全阻断。在相邻切片中通过波形蛋白和脯氨酰4-羟化酶免疫染色，将SCF阳性细胞鉴定为成纤维细胞群体。在翼状胬肉头部和体部的上皮下结缔组织以及正常结膜中未观察到明显的SCF免疫反应性。
干细胞因子在大多数翼状胬肉头部区域的成纤维细胞中过度表达。
