用新城疫病毒免疫的小鼠中病毒特异性抗体形成细胞的检测。
Detection of virus-specific antibody-forming cells of mice immunized with Newcastle disease virus.
作者信息
Kim Y T, Ault J, Wodzinski R J
出版信息
Infect Immun. 1975 May;11(5):991-5. doi: 10.1128/iai.11.5.991-995.1975.
Abstract
The hemolytic plaque assay was adapted to the detection of antibodies to Newcastle disease virus (NDV) in an in vivo, an in vitro system, and a combined in vivo-in vitro system. Several conditions were tested for coupling of sheep erythrocytes to NDV and for the kinetics of plaque formation in the in vivo and in vitro systems. The one set of conditions which provided the best responses is presented. The effect of multiple injections of NDV into mice on plaque formation was optimized.
摘要
溶血空斑试验被应用于在体内、体外系统以及体内-体外联合系统中检测新城疫病毒（NDV）抗体。针对绵羊红细胞与NDV的偶联以及体内和体外系统中斑块形成的动力学测试了多种条件。给出了一组产生最佳反应的条件。优化了向小鼠多次注射NDV对斑块形成的影响。
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