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体外对正常小鼠脾细胞悬液进行免疫接种。

Immunization of normal mouse spleen cell suspensions in vitro.

作者信息

Mishell R I, Dutton R W

出版信息

Science. 1966 Aug 26;153(3739):1004-6. doi: 10.1126/science.153.3739.1004.

DOI:10.1126/science.153.3739.1004
PMID:5917547
Abstract

Dissociated cells from the spleens of unimmunized mice were cultured with and without various mammalian erythrocytes. Spleen cell suspensions cultured with heterologous red cells developed levels of hemolytic plaque-forming cells only one log(2) less than those seen in vivo. The reaction is specific for the in vitro immunizing erythrocytes. Antibody was demonstrated in the culture fluids.

摘要

将未免疫小鼠脾脏的解离细胞与各种哺乳动物红细胞一起培养或不与红细胞一起培养。与异源红细胞一起培养的脾细胞悬液产生的溶血空斑形成细胞水平仅比体内观察到的低一个对数（2）。该反应对体外免疫红细胞具有特异性。在培养液中证实了抗体的存在。

相似文献

1
Immunization of normal mouse spleen cell suspensions in vitro.体外对正常小鼠脾细胞悬液进行免疫接种。
Science. 1966 Aug 26;153(3739):1004-6. doi: 10.1126/science.153.3739.1004.
2
Immunization of dissociated spleen cell cultures from normal mice.对来自正常小鼠的脾细胞解离培养物进行免疫接种。
J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.
3
THE MECHANISM OF TOLERANCE PRODUCED IN RATS TO SHEEP ERYTHROCYTES. I. PLAQUE-FORMING CELL AND ANTIBODY RESPONSE TO SINGLE AND MULTIPLE INJECTIONS OF ANTIGEN.大鼠对绵羊红细胞产生耐受性的机制。I. 对单次和多次注射抗原的噬斑形成细胞及抗体反应。
J Exp Med. 1965 May 1;121(5):671-81. doi: 10.1084/jem.121.5.671.
4
THE MECHANISM OF TOLERANCE PRODUCED IN RATS TO SHEEP ERYTHROCYTES. II. THE PLAQUE-FORMING CELL AND ANTIBODY RESPONSE TO MULTIPLE INJECTIONS OF ANTIGEN BEGUN AT BIRTH.大鼠对绵羊红细胞产生耐受性的机制。II. 对出生时开始多次注射抗原的空斑形成细胞及抗体反应
J Exp Med. 1965 May 1;121(5):683-95. doi: 10.1084/jem.121.5.683.
5
Cellular differentiation of the immune system of mice. II. Frequency of unipotent splenic antigen-sensitive units after immunization with sheep erythrocytes.小鼠免疫系统的细胞分化。II. 用绵羊红细胞免疫后单能脾抗原敏感单位的频率。
J Exp Med. 1969 Jan 1;129(1):185-99. doi: 10.1084/jem.129.1.185.
6
Immune responses in vitro. 3. Development of primary gamma-M, gamma-G, and gamma-A plaque-forming cell responses in mouse spleen cell cultures stimulated with heterologous erythrocytes.体外免疫反应。3. 用异种红细胞刺激小鼠脾细胞培养物中主要γ-M、γ-G和γ-A斑块形成细胞反应的发展。
J Exp Med. 1971 Aug 1;134(2):395-416. doi: 10.1084/jem.134.2.395.
7
ANTIBODY PLAQUE FORMATION BY NORMAL MOUSE SPLEEN CELL CULTURES EXPOSED IN VITRO TO RNA FROM IMMUNE MICE.体外暴露于免疫小鼠RNA的正常小鼠脾细胞培养物形成抗体斑块
Science. 1964 Nov 13;146(3646):934-6. doi: 10.1126/science.146.3646.934.
8
INHIBITION OF ANTIBODY PLAQUE FORMATION BY SENSITIZED LYMPHOID CELLS: RAPID INDICATOR OF TRANSPLANTATION IMMUNITY.致敏淋巴细胞对抗体斑块形成的抑制作用：移植免疫的快速指标。
Science. 1964 Aug 7;145(3632):607-9. doi: 10.1126/science.145.3632.607.
9
ANTIBODY PRODUCTION BY NONIMMUNE SPLEEN CELLS INCUBATED WITH RNA FROM IMMUNIZED MICE.用免疫小鼠的RNA孵育非免疫脾细胞产生抗体
Science. 1964 May 22;144(3621):1012-3. doi: 10.1126/science.144.3621.1012.
10
Cell populations and cell proliferation in the in vitro response of normal mouse spleen to heterologous erythrocytes. Analysis by the hot pulse technique.正常小鼠脾脏对异种红细胞体外反应中的细胞群体与细胞增殖。热脉冲技术分析
J Exp Med. 1967 Sep 1;126(3):443-54. doi: 10.1084/jem.126.3.443.

引用本文的文献

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The Story Behind "A Requirement for Two Cell Types for Antibody Formation in vitro".
“体外抗体形成需要两种细胞类型”背后的故事
Front Immunol. 2014 Oct 17;5:495. doi: 10.3389/fimmu.2014.00495. eCollection 2014.
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Beyond oxidative stress: an immunologist's guide to reactive oxygen species.超越氧化应激：免疫学家眼中的活性氧。
Nat Rev Immunol. 2013 May;13(5):349-61. doi: 10.1038/nri3423.
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Kicking off adaptive immunity: the discovery of dendritic cells.开启适应性免疫：树突状细胞的发现
J Exp Med. 2006 Jul 10;203(7):1622. doi: 10.1084/jem.2037fta.
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Presence of a tumour-inhibiting factor (TIF) in sera from normal but not tumour-bearing mice.正常小鼠而非荷瘤小鼠血清中存在肿瘤抑制因子（TIF）。
Immunology. 1980 Oct;41(2):289-96.
7
Further study on the humoral response of a highly-purified spleen-derived immunosuppressive peptide (SDIP).对高度纯化的脾脏来源免疫抑制肽（SDIP）体液反应的进一步研究。
Immunology. 1981 Oct;44(2):249-55.
8
Mechanisms of idiotype suppression. IV. Functional neutralization in mixtures of idiotype-specific suppressor and hapten-specific suppressor T cells.独特型抑制的机制。IV. 独特型特异性抑制性T细胞与半抗原特异性抑制性T细胞混合物中的功能中和作用。
J Exp Med. 1981 Sep 1;154(3):809-20. doi: 10.1084/jem.154.3.809.
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Anti-phenyltrimethylamino immunity in mice. II. L-Tyrosine-p-azophenyltrimethylammonium-induced suppressor T cells selectively inhibit the expression of B-cell clones bearing a cross-reactive idiotype.小鼠中的抗苯基三甲基氨基免疫。II. L-酪氨酸对偶氮苯基三甲基铵诱导的抑制性T细胞选择性抑制带有交叉反应性独特型的B细胞克隆的表达。
J Exp Med. 1980 Mar 1;151(3):528-41. doi: 10.1084/jem.151.3.528.
10
Quantitative studies on T cell diversity. III. Limiting dilution analysis of precursor cells for T helper cells reactive to xenogeneic erythrocytes.T细胞多样性的定量研究。III. 对异种红细胞反应性T辅助细胞前体细胞的有限稀释分析。
J Exp Med. 1982 Dec 1;156(6):1587-603. doi: 10.1084/jem.156.6.1587.