Simpson G, Sinkinson G, Wheelock J V
J Dairy Res. 1975 Feb;42(1):101-9. doi: 10.1017/s0022029900015132.
A comparison has been made of a number of different elution conditions for the separation by ion-exchange chromatography of the glycopeptides released by the action of rennin on whole milk. It was found that the most satisfactory separation was achieved using an NaCl gradient (0.10-0.18 M) in 0.049 M-KH2PO4+0.001 M-Na2HPO4 at a constant pH of 5.1.
已对多种不同的洗脱条件进行了比较，以便通过离子交换色谱法分离凝乳酶作用于全脂牛奶所释放的糖肽。结果发现，在恒定pH值5.1的条件下，使用0.049 M - KH₂PO₄ + 0.001 M - Na₂HPO₄中的NaCl梯度（0.10 - 0.18 M）可实现最令人满意的分离。
