Yokota Y, Sato S, Yokota M, Ishikawa Y, Makita M, Asada T, Araki Y
Yokota Ob/Gyn Clinic, Shimokoide, Maebashi, Gunma, Japan.
Hum Reprod. 2000 Aug;15(8):1802-3. doi: 10.1093/humrep/15.8.1802.
A 32 year old woman and her 32 year old spouse were referred to our IVF programme. Following recovery of 12 mature oocytes, nine were fertilized following conventional IVF. Three fresh embryos were transferred to the uterus, but all failed to result in pregnancy. Six supernumerary embryos were cultured in vitro until day 5 in order to create blastocysts. Two grew to the blastocyst stage and were vitrified using a modification of a previous method. Two blastocysts survived the freeze-thaw process and were transferred to the patient's uterus during a natural cycle, 3 months after the previous retrieval cycle. Implantation resulted in a healthy pregnancy; delivery is expected in June 2000. This report documents the first successful pregnancy in Japan, achieved via blastocyst vitrification.
一名32岁的女性及其32岁的配偶被转诊至我们的体外受精项目。在获取了12枚成熟卵母细胞后，9枚在常规体外受精后成功受精。3枚新鲜胚胎被移植到子宫，但均未成功妊娠。6枚多余的胚胎在体外培养至第5天以形成囊胚。2枚发育到囊胚阶段，并采用改良的先前方法进行玻璃化冷冻。2枚囊胚在冻融过程中存活下来，并于前一个取卵周期3个月后的自然周期被移植到患者子宫。着床成功，实现了健康妊娠；预计2000年6月分娩。本报告记录了日本首例通过囊胚玻璃化冷冻实现的成功妊娠。
