蛋白质导入叶绿体的第二个、依赖底物的位点。
A second, substrate-dependent site of protein import into chloroplasts.
作者信息
Reinbothe S, Mache R, Reinbothe C
机构信息
Université Joseph Fourier et Centre National de la Recherche Scientifique, Grenoble, France.
出版信息
Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9795-800. doi: 10.1073/pnas.160242597.
Abstract
Chloroplasts must import a large number of proteins from the cytosol. It generally is assumed that this import proceeds for all stromal and thylakoid proteins in an identical manner and is caused by the operation of two distinctive protein import machineries in the outer and inner plastid envelope, which form the general import site. Here we show that there is a second site of protein translocation into chloroplasts of barley, tobacco, Arabidopsis thaliana, and five other tested monocotyledonous and dicotyledonous plant species. This import site is specific for the cytosolic precursor of the NADPH:protochlorophyllide (Pchlide) oxidoreductase A, pPORA. It couples Pchlide synthesis to pPORA import and thereby reduces the actual level of free Pchlide, which, because of its photodynamic properties, would be destructive to the plastids. Consequently, photoprotection is conferred onto the plant.
摘要
叶绿体必须从细胞质中导入大量蛋白质。通常认为，所有基质蛋白和类囊体蛋白的导入方式相同，且是由质体包膜外层和内层中两种不同的蛋白质导入机制共同作用引起的，这两种机制构成了一般导入位点。在此，我们表明，在大麦、烟草、拟南芥以及其他五种经测试的单子叶和双子叶植物物种的叶绿体中，存在第二个蛋白质转运位点。该导入位点对NADPH：原叶绿素酸酯（Pchlide）氧化还原酶A（pPORA）的胞质前体具有特异性。它将Pchlide的合成与pPORA的导入耦合在一起，从而降低了游离Pchlide的实际水平，由于其光动力学特性，游离Pchlide会对质体造成破坏。因此，植物获得了光保护。
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本文引用的文献
1
In-vitro transport of chloroplast proteins in a homologousEuglena system with particular reference to plastid leucyl-tRNA synthetase.叶绿体蛋白在同源衣藻系统中的体外转运，特别参考了质体亮氨酰-tRNA 合成酶。
Planta. 1990 May;181(2):176-83. doi: 10.1007/BF02411535.
2
Chloroplast protein import : quantitative analysis of precursor binding.叶绿体蛋白导入：前体结合的定量分析。
Plant Physiol. 1989 Mar;89(3):993-9. doi: 10.1104/pp.89.3.993.
3
Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts.嗜热菌蛋白酶是一种用于探测完整豌豆叶绿体表面的合适蛋白酶。
Plant Physiol. 1984 Jul;75(3):675-8. doi: 10.1104/pp.75.3.675.
4
Protein Import into and Sorting inside the Chloroplast Are Independent Processes.蛋白质输入叶绿体及在叶绿体内的分选是独立过程。
Plant Cell. 1990 May;2(5):479-494. doi: 10.1105/tpc.2.5.479.
5
Two NADPH:Protochlorophyllide Oxidoreductases in Barley: Evidence for the Selective Disappearance of PORA during the Light-Induced Greening of Etiolated Seedlings.大麦中的两种NADPH：原叶绿素酸酯氧化还原酶：黄化幼苗光诱导变绿过程中PORA选择性消失的证据。
Plant Cell. 1995 Nov;7(11):1933-1940. doi: 10.1105/tpc.7.11.1933.
6
Mechanism of Protein Transport across the Chloroplast Envelope.蛋白质跨叶绿体包膜运输的机制
Plant Physiol. 1997 Jun;114(2):405-410. doi: 10.1104/pp.114.2.405.
7
A plastid enzyme arrested in the step of precursor translocation in vivo.一种在体内前体转运步骤中被阻断的质体酶。
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):12026-30. doi: 10.1073/pnas.93.21.12026.
8
Regulation of chloroplast protein import through a protochlorophyllide-responsive transit peptide.通过原叶绿素酸酯响应转运肽对叶绿体蛋白质输入的调控。
Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8890-4. doi: 10.1073/pnas.94.16.8890.
9
An Arabidopsis mutant defective in the plastid general protein import apparatus.一种质体通用蛋白质输入装置存在缺陷的拟南芥突变体。
Science. 1998 Oct 2;282(5386):100-3. doi: 10.1126/science.282.5386.100.
10
Analysis of the interactions of preproteins with the import machinery over the course of protein import into chloroplasts.在蛋白质导入叶绿体的过程中，对前体蛋白与导入机制之间相互作用的分析。
J Cell Biol. 1997 Dec 29;139(7):1677-85. doi: 10.1083/jcb.139.7.1677.
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