Kügler S, Schurtz Sebghati T, Groppe Eissenberg L, Goldman W E
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8794-8. doi: 10.1073/pnas.97.16.8794.
The success of Histoplasma capsulatum as an intracellular pathogen depends completely on successful conversion of the saprophytic mycelial (mold) form of this fungus to a parasitic yeast form. It is therefore not surprising that yeast phase-specific genes and gene products are proving to be important for survival and proliferation of H. capsulatum within macrophages. In this study, we have focused on the role and regulation of two yeast-specific characteristics: alpha-(1,3)-glucan, a cell wall polysaccharide modulated by cell-density (quorum) sensing, and a secreted calcium-binding protein (CBP) that is essential for pathogenicity.
荚膜组织胞浆菌作为一种细胞内病原体的成功完全取决于该真菌的腐生菌丝体(霉菌)形式成功转化为寄生酵母形式。因此,酵母阶段特异性基因和基因产物对荚膜组织胞浆菌在巨噬细胞内的存活和增殖至关重要也就不足为奇了。在本研究中,我们重点关注了两个酵母特异性特征的作用和调控:α-(1,3)-葡聚糖,一种受细胞密度(群体)感应调节的细胞壁多糖,以及一种对致病性至关重要的分泌型钙结合蛋白(CBP)。