Sailendri K, Muthukkaruppan V R
J Exp Zool. 1975 Mar;191(3):371-82. doi: 10.1002/jez.1401910308.
The immune response of Tilapia mossambica to bovine serum albumin (BSA) and sheep red blood cells (SRBC) was characterized in detail in terms of the appearance of hemolysin plaque-forming cells and circulating antibodies at 30 degrees C. Plaque-forming cells (PFC) were detected in the spleen, head-kidney and thymus of immunized fish and the maximum number was observed in these organs on the fifth day after immunization with SRBC. Peak circulating antibody response occurred on day 8 for SRBC and on day 11 for BSA. Following the second injection of the same antigen, a specific anamnestic response was observed with increased production of PFC and serum antibody. No cross reactivity was found when anti-SRBC antibody was tested with rat erythrocytes. Tests with 2-mercaptoethanol showed that all of the agglutinating antibody produced after both the first and second injection was mercaptoethanol sensitive. Analysis of histological and smear preparations revealed that there were consistent cellular changes occurring in the spleen as well as the head-kidney due to immunization.
在30摄氏度条件下，详细研究了莫桑比克罗非鱼对牛血清白蛋白（BSA）和绵羊红细胞（SRBC）的免疫反应，包括溶血素斑块形成细胞的出现情况和循环抗体。在免疫鱼的脾脏、头肾和胸腺中检测到斑块形成细胞（PFC），在用SRBC免疫后的第5天，在这些器官中观察到最大数量的PFC。SRBC的循环抗体反应高峰出现在第8天，BSA的循环抗体反应高峰出现在第11天。在第二次注射相同抗原后，观察到特异性回忆反应，PFC和血清抗体的产生增加。用大鼠红细胞检测抗SRBC抗体时未发现交叉反应。用2-巯基乙醇进行的试验表明，第一次和第二次注射后产生的所有凝集抗体对巯基乙醇敏感。组织学和涂片制剂分析表明，免疫导致脾脏和头肾出现一致的细胞变化。
