Taché Y, Brown M, Collu R
Endocrinology. 1979 Jul;105(1):220-4. doi: 10.1210/endo-105-1-220.
The effects of bombesin and other unrelated oligopeptides on hormonal changes induced by stress were studied in conscious adult male rats. Restraint in the cold for 1 h increased plasma corticosterone and PRL levels and decreased GH values but had no effect on LH levels. Bombesin (5 microgram), given intracerebroventricularly (ivt) before stress, inhibited the PRL rise without affecting corticosterone, GH, or LH response. A complete blockade of PRL rise was observed with doses of bombesin ranging from 5 microgram to 100 ng ivt, regardless of the duration (15, 30, 45, or 60 min) or the nature (cold exposure or restraint at room temperature) of the stressor agents. Bombesin was 10(3) more potent as a PRL inhibitor when given ivt than when given iv, and its ivt effect was not reversed by naloxone (1 or 10 mg/kg). Among other unrelated peptides tested (beta-endorphin, neurotensin, substance P, and TRH; 5 microgram ivt), only neurotensin decreased plasma PRL levels in rats subjected to restraint in the cold for 1 h. These results show that in conscious male rats, centrally administered bombesin has a very potent and long acting inhibitory effect on PRL release induced by acute stress. Since a bombesin-like peptide has been found in rat brain, its physiological role in PRL regulation remains to be elucidated.
在清醒的成年雄性大鼠中研究了蛙皮素和其他不相关的寡肽对应激诱导的激素变化的影响。在寒冷环境中束缚1小时会使血浆皮质酮和催乳素水平升高，生长激素值降低，但对促黄体生成素水平没有影响。在应激前经脑室注射（ivt）给予蛙皮素（5微克），可抑制催乳素升高，而不影响皮质酮、生长激素或促黄体生成素的反应。无论应激源的持续时间（15、30、45或60分钟）或性质（冷暴露或室温下束缚）如何，ivt给予5微克至100纳克的蛙皮素剂量均能完全阻断催乳素升高。蛙皮素经ivt给药时作为催乳素抑制剂的效力比静脉给药时高10³倍，其ivt效应不会被纳洛酮（1或10毫克/千克）逆转。在测试的其他不相关肽（β-内啡肽、神经降压素、P物质和促甲状腺激素释放激素；5微克ivt）中，只有神经降压素能降低在寒冷中束缚1小时的大鼠的血浆催乳素水平。这些结果表明，在清醒的雄性大鼠中，经中枢给予蛙皮素对急性应激诱导的催乳素释放具有非常强效且长效的抑制作用。由于在大鼠脑中发现了一种类似蛙皮素的肽，其在催乳素调节中的生理作用仍有待阐明。
