Puławska J, Maes M, Willems A, Sobiczewski P
Research Institute of Pomology and Floriculture, Skierniewice, Poland.
Syst Appl Microbiol. 2000 Jun;23(2):238-44. doi: 10.1016/S0723-2020(00)80010-7.
The phylogenetic relationship among twelve Agrobacterium, four Rhizobium, and two Sinorhizobium strains originating from various host plants and geographical regions was studied by analysis of the 23S rDNA sequences. The study included Agrobacterium strains belonging to biovars 1, 2 (with tumor- or hairy-root inducing and non-pathogenic strains), A. vitis, A. rubi; representative species of the Rhizobium genus: R. galegae, R. leguminosarum and R. tropici and Sinorhizobium meliloti strains. The phylogenetic analysis showed that within Agrobacterium, the biovar designation was reflected in the 23S rDNA similarity and that strains of Agrobacterium and Rhizobium are closely related to each other. The results suggest that the taxonomic definition of Agrobacterium and Rhizobium should be considered for revision and that the Agrobacterium-biovar identity is probably a reliable taxonomic trait.
通过分析23S rDNA序列，研究了源自不同宿主植物和地理区域的12株农杆菌、4株根瘤菌和2株中华根瘤菌菌株之间的系统发育关系。该研究包括属于生物变种1、2（具有诱导肿瘤或毛根以及非致病菌株）的农杆菌菌株、葡萄农杆菌、悬钩子农杆菌；根瘤菌属的代表性物种：加里根瘤菌、豌豆根瘤菌和热带根瘤菌以及苜蓿中华根瘤菌菌株。系统发育分析表明，在农杆菌属内，生物变种的命名反映在23S rDNA相似性上，并且农杆菌和根瘤菌的菌株彼此密切相关。结果表明，应考虑修订农杆菌和根瘤菌的分类定义，并且农杆菌生物变种的身份可能是一个可靠的分类学特征。
