Sawada H, Ieki H, Oyaizu H, Matsumoto S
Akitsu Branch, Fruit Tree Research Station, Hiroshima, Japan.
Int J Syst Bacteriol. 1993 Oct;43(4):694-702. doi: 10.1099/00207713-43-4-694.
The 16S rRNA sequences of seven representative Agrobacterium strains, eight representative Rhizobium strains, and the type strains of Azorhizobium caulinodans and Bradyrhizobium japonicum were determined. These strains included the type strains of Agrobacterium tumefaciens, Agrobacterium rhizogenes, Agrobacterium radiobacter, Agrobacterium vitis, Agrobacterium rubi, Rhizobium fredii, Rhizobium galegae, Rhizobium huakuii, Rhizobium leguminosarum, Rhizobium loti, Rhizobium meliloti, and Rhizobium tropici. A phylogenetic analysis showed that the 15 strains of Agrobacterium and Rhizobium species formed a compact phylogenetic cluster clearly separated from the other members of the alpha subclass of the Proteobacteria. However, Agrobacterium species and Rhizobium species are phylogenetically entwined with one another, and the two genera cannot be separated. In the Agrobacterium species, the strains of biovar 1, biovar 2, Agrobacterium rubi, and Agrobacterium vitis were clearly separated. The two biovars exhibited homogeneity in their phenotypic, chemotaxonomic, and phylogenetic characteristics, and two species should be established for the two biovars. We considered the nomenclature of the two biovars, and revised descriptions of Agrobacterium radiobacter (for the biovar 1 strains) and Agrobacterium rhizogenes (for the biovar 2 strains) are proposed. The name Agrobacterium tumefaciens is rejected because the type strain of this species was assigned to Agrobacterium radiobacter, and consequently the description of the genus Agrobacterium is revised.
测定了7株代表性土壤杆菌菌株、8株代表性根瘤菌菌株以及茎瘤固氮根瘤菌和大豆慢生根瘤菌模式菌株的16S rRNA序列。这些菌株包括根癌土壤杆菌、发根土壤杆菌、放射土壤杆菌、葡萄土壤杆菌、悬钩子土壤杆菌、费氏根瘤菌、山羊根瘤菌、华癸根瘤菌、豌豆根瘤菌、百脉根根瘤菌、热带根瘤菌的模式菌株。系统发育分析表明，15株土壤杆菌属和根瘤菌属菌株形成了一个紧密的系统发育簇，明显与变形菌门α亚纲的其他成员分开。然而，土壤杆菌属物种和根瘤菌属物种在系统发育上相互缠绕，无法将这两个属分开。在土壤杆菌属物种中，生物变种1、生物变种2、悬钩子土壤杆菌和葡萄土壤杆菌的菌株明显分开。这两个生物变种在表型、化学分类和系统发育特征上表现出同质性，应为这两个生物变种建立两个物种。我们考虑了这两个生物变种的命名法，并提出了放射土壤杆菌（针对生物变种1菌株）和发根土壤杆菌（针对生物变种2菌株）的修订描述。根癌土壤杆菌这个名称被摒弃，因为该物种的模式菌株被归为放射土壤杆菌，因此对土壤杆菌属的描述进行了修订。
