Ottesen G L, Christensen I J, Larsen J K, Larsen J, Baldetorp B, Linden T, Hansen B, Andersen J
Department of Forensic Pathology, University of Copenhagen.
Breast Cancer Res Treat. 2000 Apr;60(3):219-26. doi: 10.1023/a:1006453420088.
In a consecutive and unselected series of 178 cases of carcinoma in situ of the breast (CIS), comprising both ductal (DCIS) and lobular type (LCIS), and a series of 48 cases of invasive carcinoma (IC) with predominance of DCIS, the association between histopathology, immunohistochemical markers (ER, PgR, MIB-1, c-erbB-2, and p53), and DNA ploidy was investigated, in order to discriminate biologically different groups. In DCIS, significant correlation was shown between large nuclear size and comedonecrosis, both of which showed also strong association to DNA aneuploidy, high proliferation activity, low steroid receptor content, and overexpression of c-erbB-2 and p53 factors that may indicate an aggressive behavior. Small nuclear CIS, whether LCIS or DCIS, on the contrary, were DNA diploid with low proliferation, and no cases showed overexpression of c-erbB-2 and p53. Heterogeneity with respect to the investigated parameters was also a frequent finding that may reflect a development complexity. In IC, comparison of the DCIS and the invasive component showed similar patterns. No significant differences were shown between DCIS without and with invasion. This may indicate that none of the investigated parameters on its own are essential for the event of invasion.
在一组连续且未经筛选的178例乳腺原位癌（CIS）病例中，包括导管原位癌（DCIS）和小叶原位癌（LCIS），以及一组以DCIS为主的48例浸润性癌（IC）病例中，研究了组织病理学、免疫组化标志物（ER、PgR、MIB-1、c-erbB-2和p53）与DNA倍体之间的关联，以区分生物学上不同的组。在DCIS中，细胞核大与粉刺样坏死之间存在显著相关性，二者均与DNA非整倍体、高增殖活性、低类固醇受体含量以及可能表明侵袭性行为的c-erbB-2和p53因子过表达也密切相关。相反，小细胞核CIS，无论是LCIS还是DCIS，均为DNA二倍体，增殖率低，且无病例显示c-erbB-2和p53过表达。在所研究参数方面的异质性也是一个常见发现，这可能反映了其发展的复杂性。在IC中，DCIS与浸润成分的比较显示出相似的模式。有浸润和无浸润的DCIS之间未显示出显著差异。这可能表明所研究的参数单独一项对于浸润事件都不是必需的。
