Maeda N, Kon K, Tateishi N, Suzuki Y, Sekiya M, Taniguchi T, Seike M, Nakajima T, Shiga T, Tanaka K
Department of Physiology, School of Medicine, Ehime University, Japan.
Br J Haematol. 1989 Sep;73(1):105-11. doi: 10.1111/j.1365-2141.1989.tb00228.x.
Recombinant human erythropoietin [rhEPO (180 iu/micrograms); 1 or 10 micrograms polypeptide equivalent/kg] was intravenously administered daily to 6-week-old normal rats for 1 week. Rheologically, (1) blood viscosity at shear rate of 40-380 s-1 increased in a manner entirely dependent upon the haematocrit, (2) no change in erythrocyte deformability was detected by high shear rheoscopy, and (3) the velocity of rouleau formation in autologous plasma (at 7.5 s-1) decreased. Haematologically, rhEPO administration induced considerable polycythaemia with reticulocytosis in a dose-dependent manner, accompanying increased cell volume and decreased intracellular haemoglobin concentration, thus the density distribution of erythrocytes shifted towards low specific gravity. Plasma viscosity and plasma protein composition were unaffected by rhEPO-administration. In erythrocyte metabolism, no drastic alteration in the level of 2.3-DPG or ATP was detected.
将重组人促红细胞生成素[rhEPO（180国际单位/微克）；1或10微克多肽当量/千克]每天静脉注射给6周龄正常大鼠，持续1周。在流变学方面，（1）剪切速率为40 - 380 s-1时的血液粘度增加，其方式完全取决于血细胞比容，（2）通过高剪切流变学未检测到红细胞变形性的变化，（3）自体血浆中（在7.5 s-1）缗钱状形成速度降低。在血液学方面，rhEPO给药以剂量依赖的方式诱导了明显的红细胞增多症并伴有网织红细胞增多，同时细胞体积增加且细胞内血红蛋白浓度降低，因此红细胞的密度分布向低比重转移。血浆粘度和血浆蛋白组成不受rhEPO给药的影响。在红细胞代谢方面，未检测到2,3 - DPG或ATP水平的剧烈变化。
