染色质对DNA聚合酶I的可及性以及F1组蛋白的定位
Accessibility of chromatin to DNA polymerase I and location of the F1 histone.
作者信息
Saffhill R, Itzhaki R F
出版信息
Nucleic Acids Res. 1975 Jan;2(1):113-9. doi: 10.1093/nar/2.1.113.
The effect of prebound poly-L-lysine upon the template activity of DNA, chromatin and F1 histone-depleted chromatin for E. coli DNA polymerase I has been investigated. Measurements have been made in the absence and presence of 0.1M NaCl. From the results we conclude that only ca 60% of the polylysine-accessible DNA, i.e. 22% of the total DNA of the chromatin, is accessible to the DNA polymerase I and that ca. 30% of the polylysine-accessible DNA, i.e. 11% of the total DNA, is associated with the F1 histone.
已研究了预先结合的聚-L-赖氨酸对DNA、染色质以及F1组蛋白缺失染色质作为大肠杆菌DNA聚合酶I模板活性的影响。在不存在和存在0.1M NaCl的情况下进行了测量。根据结果我们得出结论，只有约60%的聚赖氨酸可及DNA，即染色质总DNA的22%，可被DNA聚合酶I所利用，并且约30%的聚赖氨酸可及DNA，即总DNA的11%，与F1组蛋白相关联。
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