Di Cesare A, Paris S, Albertinazzi C, Dariozzi S, Andersen J, Mann M, Longhi R, de Curtis I
Cell Adhesion Unit - DIBIT - S. Raffaele Scientific Institute, Via Olgettina 58, 20132 Milano, Italy.
Nat Cell Biol. 2000 Aug;2(8):521-30. doi: 10.1038/35019561.
Motility requires protrusive activity at the cellular edge, where Rho family members regulate actin dynamics. Here we show that p95-APP1 (ArfGAP-putative, Pix-interacting, paxillin-interacting protein 1), a member of the GIT1/PKL family, is part of a complex that interacts with Rac. Wild-type and truncated p95-APP1 induce actin-rich protrusions mediated by Rac and ADP-ribosylation factor 6 (Arf6). Distinct p95-APP1-derived polypeptides have different distributions, indicating that p95-APP1 cycles between the cell surface and endosomes. Our results show that p95-APP1 functionally interacts with Rac and localizes to endosomal compartments, thus identifying p95-APP1 as a molecular link between actin organization, adhesion, and membrane transport during cell motility.
细胞运动需要细胞边缘的突出活动，其中Rho家族成员调节肌动蛋白动力学。我们在此表明，p95-APP1（一种假定的ArfGAP、与Pix相互作用、与桩蛋白相互作用的蛋白1）是GIT1/PKL家族的成员，是与Rac相互作用的复合物的一部分。野生型和截短的p95-APP1诱导由Rac和ADP-核糖基化因子6（Arf6）介导的富含肌动蛋白的突出。不同的p95-APP1衍生多肽具有不同的分布，表明p95-APP1在细胞表面和内体之间循环。我们的结果表明，p95-APP1在功能上与Rac相互作用并定位于内体区室，从而将p95-APP1鉴定为细胞运动过程中肌动蛋白组织、黏附与膜运输之间的分子联系。
