Alvarez-Díaz C, Cuenca-Pardo J, Sosa-Serrano A, Juárez-Aguilar E, Marsch-Moreno M, Kuri-Harcuch W
Burn Unit, Hospital de Traumatología Victorio de la Fuente Narvaez, IMSS, Mexico City, Mexico.
J Burn Care Rehabil. 2000 Jul-Aug;21(4):291-9. doi: 10.1067/mbc.2000.106393.
Numerous studies, many uncontrolled, have suggested that the application of freshly prepared human allogeneic epidermal cultures promotes faster re-epithelialization of skin donor sites and deep partial-thickness burns. We describe the results of a study of deep partial-thickness burns treated with such cultures preserved in the frozen state. The study was controlled, side-by-side comparative, and randomized. Nine patients with deep partial-thickness burns and 2 patients with superficial partial-thickness burns were treated with the frozen cultures, with the use of adjacent wounds covered with petrolatum-coated gauze (Jelonet, Smith & Nephew Inc, Largo, Fla) as control wounds. The results showed that for the 2 superficial partial-thickness burns, the frozen cultures reduced healing time by 44%. For 5 of the patients with deep partial-thickness burns, the wounds treated with frozen cultures healed in a mean time of 5.6 days, whereas the control wounds healed in 12.2 days. More importantly, for the 4 other patients with deep partial-thickness burns, the wounds treated with the frozen cultures underwent complete re-epithelialization in a mean time of 4.2 days, but the control wounds were partially or mostly unhealed at up to 14 days. The results show that the frozen cultures not only accelerate the re-epithelialization of deep and superficial partial-thickness burns but also make it possible to heal such wounds that otherwise would not heal.
许多研究（其中许多未设对照）表明，应用新鲜制备的人异体表皮培养物可促进皮肤供皮区和深度Ⅱ度烧伤更快地重新上皮化。我们描述了一项关于用冷冻保存的此类培养物治疗深度Ⅱ度烧伤的研究结果。该研究为对照、并列比较且随机化的研究。9例深度Ⅱ度烧伤患者和2例浅Ⅱ度烧伤患者接受了冷冻培养物治疗，相邻伤口用涂有凡士林的纱布（Jelonet，施乐辉公司，拉戈，佛罗里达州）覆盖作为对照伤口。结果显示，对于2例浅Ⅱ度烧伤患者，冷冻培养物使愈合时间缩短了44%。对于5例深度Ⅱ度烧伤患者，用冷冻培养物治疗的伤口平均在5.6天愈合，而对照伤口在12.2天愈合。更重要的是，对于另外4例深度Ⅱ度烧伤患者，用冷冻培养物治疗的伤口平均在4.2天完全重新上皮化，但对照伤口在长达14天时部分或大部分未愈合。结果表明，冷冻培养物不仅能加速深度和浅Ⅱ度烧伤的重新上皮化，还能使原本无法愈合的此类伤口得以愈合。
