Vdovenko A A, Williams J E
University of Hawaii, Dept. of Pathology, Honolulu, 96822, USA.
Parasitol Res. 2000 Jul;86(7):573-81. doi: 10.1007/pl00008533.
A simple in vitro drug sensitivity testing system for Blastocystis hominis clinical isolates was developed. Application of supravital staining by neutral red allowed quantitative viability assessment. Four xenic cultures, isolated from human sources, were grown in modified monophasic Robinson's medium and tested for sensitivities to nine available drugs. Assessment was done using the cell-count method from air-dried preparations after supravital staining with neutral red. Also, the light absorbence method was evaluated. Trimethoprim, metronidazole, quinacrine, tetracycline, paromomycin, and two new antiprotozoal drugs, nitazoxanide and deacetyl-nitazoxanide, showed cytostatic or cytocidal effects on all or some Blastocystis isolates. Chloroquine and sulphamethoxazole did not demonstrate any effect at the concentrations studied.
开发了一种用于人芽囊原虫临床分离株的简单体外药敏试验系统。通过中性红进行活染可进行定量活力评估。从人类来源分离出的四种异源培养物在改良的单相罗宾逊培养基中培养，并测试了对九种现有药物的敏感性。在用中性红进行活染后，通过细胞计数法对风干制剂进行评估。此外，还对吸光法进行了评估。甲氧苄啶、甲硝唑、奎纳克林、四环素、巴龙霉素以及两种新型抗原虫药物硝唑尼特和去乙酰硝唑尼特对所有或部分人芽囊原虫分离株显示出细胞抑制或细胞杀伤作用。在所研究的浓度下，氯喹和磺胺甲恶唑未显示出任何作用。
