Haresh K, Suresh K, Khairul Anus A, Saminathan S
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Trop Med Int Health. 1999 Apr;4(4):274-7. doi: 10.1046/j.1365-3156.1999.00398.x.
Isolates of Blastocystis hominis from infected immigrant workers from Indonesia, Bangladesh and infected individuals from Singapore and Malaysia were assessed for growth pattern and degree of resistance to different concentrations of metronidazole. Viability of the cells was assessed using eosin-brillian cresyl blue which stained viable cells green and nonviable cells red. The Bangladeshi and Singaporean isolates were nonviable even at the lowest concentration of 0.01 mg/ml, whereas 40% of the initial inoculum of parasites from the Indonesian isolate at day one were still viable in cultures with 1.0 mg/ml metronidazole. The study shows that isolates of B. hominis of different geographical origin have different levels of resistance to metronidazole. The search for more effective drugs to eliminate th parasite appears inevitable, especially since surviving parasites from metronidazole cultures show greater ability to multiply in subcultures than controls.
对来自印度尼西亚、孟加拉国的受感染移民工人以及来自新加坡和马来西亚的受感染个体的人芽囊原虫分离株进行了生长模式和对不同浓度甲硝唑耐药程度的评估。使用伊红-灿烂甲酚蓝评估细胞活力，该染料将活细胞染成绿色，死细胞染成红色。孟加拉国和新加坡的分离株即使在最低浓度0.01mg/ml时也无活力，而来自印度尼西亚分离株的初始接种寄生虫在第1天，有40%在含有1.0mg/ml甲硝唑的培养物中仍有活力。该研究表明，不同地理来源的人芽囊原虫分离株对甲硝唑具有不同程度的耐药性。寻找更有效的药物来消除这种寄生虫似乎是不可避免 的，特别是因为来自甲硝唑培养物中的存活寄生虫在传代培养中比对照显示出更强的增殖能力。
