Santangelo G, Bruno P
Dipartimento Etologia, Ecologia, Evoluzione, Via Volta N6, I-56126 Pisa, Italy.
Micron. 2001 Feb;32(2):207-10. doi: 10.1016/s0968-4328(99)00144-4.
Ciliated protozoans represent useful organisms for studying the processes involved in the induction and progression of meiosis. In this short report we describe a technique that has allowed us to examine different meiosis phases during the sexual reproduction of Blepharisma japonicum. In order to visualize the phases of meiosis, sexually reproducing pairs were stained by an enhanced technique of anti-tubuline indirect immunofluorescence. Meiotic micronuclei, particularly those showing metaphase spindles, were clearly highlighted. The technique also heavily decorates the main microtubular cytoplasmic arrays in Blepharisma.
纤毛原生动物是研究减数分裂诱导和进程相关过程的有用生物体。在本简短报告中，我们描述了一种技术，该技术使我们能够在日本大草履虫有性繁殖期间检查不同的减数分裂阶段。为了观察减数分裂的各个阶段，通过抗微管蛋白间接免疫荧光增强技术对进行有性繁殖的草履虫对进行染色。减数分裂微核，特别是那些显示中期纺锤体的微核，被清晰地突显出来。该技术还能显著地标记大草履虫主要的微管细胞质阵列。
