de Oliveira S A, Siqueira M M, Brown D W, Litton P, Camacho L A, Castro S T, Cohen B J
Disciplina de Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brasil.
Rev Soc Bras Med Trop. 2000 Jul-Aug;33(4):335-9. doi: 10.1590/s0037-86822000000400001.
This study was designed to investigate whether saliva could be a feasible alternative to serum for the diagnosis of recent rubella infection in a clinic setting. Forty-five paired blood and saliva samples collected 1 to 29 days after onset of illness were tested for specific immunoglobulin (Ig) M by antibody-capture radioimmunoassay (MACRIA). Rubella IgM was detected in all serum samples and in 38 (84.4%) saliva specimens. Forty-six serum and saliva samples from other patients with rash diseases were tested by MACRIA for control purposes and two saliva specimens were reactive. The saliva test had specificity of 96%. These results indicate that salivary IgM detection may be a convenient non-invasive alternative to serum for investigation of recent rubella cases, especially for disease surveillance and control programmes.
本研究旨在调查在临床环境中，唾液是否可作为血清的可行替代物用于诊断近期风疹感染。对发病后1至29天采集的45对血液和唾液样本，采用抗体捕获放射免疫分析（MACRIA）检测特异性免疫球蛋白（Ig）M。所有血清样本及38份（84.4%）唾液标本中均检测到风疹IgM。为作对照，采用MACRIA检测了其他出疹性疾病患者的46份血清和唾液样本，两份唾液标本呈反应性。唾液检测的特异性为96%。这些结果表明，检测唾液IgM可能是一种方便的非侵入性替代方法，可用于调查近期风疹病例，特别是用于疾病监测和控制项目。
