de Azevedo Neto R S, Richards A, Nokes D J, Silveira A S, Cohen B J, Passos S D, de Souza V A, Brown D W, Pannuti C S, Massad E
Department of Pathology, Faculty of Medicine, University of São Paulo, Brazil.
Trans R Soc Trop Med Hyg. 1995 Jan-Feb;89(1):115-8. doi: 10.1016/0035-9203(95)90680-0.
The sensitivity and specificity of salivary rubella antibody detection was investigated using samples collected from 301 children after a mass vaccination campaign in the state of São Paulo, Brazil. Saliva samples were collected by 2 different methods: directly dribbling into a container or using a commercial collecting device. Corresponding finger-prick blood samples were collected on filter paper. Rubella specific immunoglobulin G (IgG) was measured in saliva by antibody capture radioimmunoassay and in blood samples by indirect enzyme-linked immunosorbent assay. The detection of salivary rubella specific IgG showed good correlation with the detection of rubella antibody in the blood samples. For both collecting techniques the predictive value for a positive saliva test was > 99% compared with the results from the blood tests. However, the predictive value for a negative saliva test was only 58.3% for a dribbled sample, compared to 100% for saliva collected using the commercial device. Moreover, collecting saliva by dribbling from children less than 4 years old was difficult. The detection of rubella specific IgG in saliva collected using a commercial device proved to be sensitive and specific in this epidemiological study, encouraging its more widespread application as a means of surveillance after mass vaccination.
利用从巴西圣保罗州大规模疫苗接种运动后的301名儿童中采集的样本，对唾液风疹抗体检测的敏感性和特异性进行了研究。唾液样本通过两种不同方法采集：直接滴入容器或使用商用采集装置。相应的手指刺血样本采集在滤纸上。通过抗体捕获放射免疫分析法测定唾液中的风疹特异性免疫球蛋白G（IgG），通过间接酶联免疫吸附测定法测定血样中的风疹特异性免疫球蛋白G。唾液中风疹特异性IgG的检测与血样中风疹抗体的检测显示出良好的相关性。对于两种采集技术，唾液检测呈阳性的预测值与血液检测结果相比均>99%。然而，对于直接滴入的样本，唾液检测呈阴性的预测值仅为58.3%，而使用商用装置采集的唾液样本的预测值为100%。此外，从4岁以下儿童中直接滴入唾液进行采集很困难。在这项流行病学研究中，使用商用装置采集的唾液中风疹特异性IgG的检测被证明具有敏感性和特异性，这鼓励将其更广泛地用作大规模疫苗接种后监测的一种手段。
