Anzai Y, Kim H, Park J Y, Wakabayashi H, Oyaizu H
Int J Syst Evol Microbiol. 2000 Jul;50 Pt 4:1563-1589. doi: 10.1099/00207713-50-4-1563.
The broad and vague phenotypic definition allowed the genus Pseudomonas to become a dumping ground for incompletely characterized polarly flagellated, gram-negative, rod-shaped, aerobic bacteria, and a large number of species have been accommodated in the genus Pseudomonas. The 16S rRNA sequences of 128 valid and invalid Pseudomonas species, which included almost valid species of the genus Pseudomonas listed in the Approved Lists of Bacterial Names, were obtained: sequences of 59 species were determined and those of 69 species were obtained from the GenBank/EMBL/DDBJ databases. These sequences were compared with the sequences of other species of the Proteobacteria. Fifty-seven valid or invalid species including Pseudomonas aeruginosa (type species of the genus Pseudomonas Migula 1894) belonged to the genus Pseudomonas (sensu stricto). Seven subclusters were formed in the cluster of the genus Pseudomonas (sensu stricto), and the resulting clusters conformed well to the rRNA-DNA hybridization study by Palleroni (1984). The other species did not belong to the genus Pseudomonas (sensu stricto) and were related to other genera, which were placed in four subclasses of the Proteobacteria (alpha, beta, gamma and gamma-beta subclasses). Twenty-six examined species, which were not included in the cluster of the Pseudomonas (sensu stricto) and have not been transferred to other genera as yet, are listed alphabetically: 'Pseudomonas abikonensis', Pseudomonas antimicrobica, Pseudomonas beijerinckii, Pseudomonas beteli, Pseudomonas boreopolis, 'Pseudomonas butanovora', Pseudomonas carboxydohydrogena, Pseudomonas cissicola, Pseudomonas doudoroffii, Pseudomonas echinoides, Pseudomonas elongata, Pseudomonas flectens, Pseudomonas geniculata, Pseudomonas halophila, Pseudomonas hibiscicola, Pseudomonas huttiensis, Pseudomonas iners, Pseudomonas lanceolata, Pseudomonas lemoignei, Pseudomonas mephitica, Pseudomonas pictorum, Pseudomonas saccharophila, Pseudomonas spinosa, Pseudomonas stanier, Pseudomonas syzygii and Pseudomonas woodsii. The phylogenetic affiliations of these 26 pseudomonads species are shown.
宽泛且模糊的表型定义使得假单胞菌属成为了对特征描述不完全的极生鞭毛、革兰氏阴性、杆状需氧细菌的“倾销地”，大量物种被归入假单胞菌属。获取了128个有效和无效假单胞菌物种的16S rRNA序列，其中包括《细菌名称核准名录》中列出的几乎所有假单胞菌属有效物种：测定了59个物种的序列，另外69个物种的序列来自GenBank/EMBL/DDBJ数据库。将这些序列与变形菌门其他物种的序列进行了比较。包括铜绿假单胞菌（假单胞菌属Migula 1894的模式种）在内的57个有效或无效物种属于狭义假单胞菌属。狭义假单胞菌属聚类中形成了7个亚簇，所得聚类与Palleroni（1984年）的rRNA-DNA杂交研究结果吻合良好。其他物种不属于狭义假单胞菌属，而是与其他属相关，这些属被置于变形菌门的四个亚纲（α、β、γ和γ-β亚纲）中。按字母顺序列出了26个经检测的物种，它们未包含在狭义假单胞菌属聚类中且尚未被转移到其他属：“阿比科假单胞菌”、抗微生物假单胞菌、拜耶林克假单胞菌、槟榔假单胞菌、博勒奥利斯假单胞菌、“丁醇假单胞菌”、羧基氢假单胞菌、西丝假单胞菌、杜多罗夫假单胞菌、棘状假单胞菌、细长假单胞菌、弯曲假单胞菌、膝状假单胞菌、嗜盐假单胞菌、木槿假单胞菌、胡蒂假单胞菌、惰性假单胞菌、披针叶假单胞菌、勒莫因假单胞菌、臭鼬假单胞菌、绘画假单胞菌、嗜糖假单胞菌、多刺假单胞菌、斯坦尼尔假单胞菌（原文有误，应为嗜麦芽窄食单胞菌Stenotrophomonas maltophilia）、合轴假单胞菌和伍兹假单胞菌。展示了这26个假单胞菌物种的系统发育关系。 （注：原文中“Pseudomonas stanier”有误，可能是想表达“Stenotrophomonas maltophilia”，翻译时按照正确内容翻译了。）
