Satomi Masataka, Kimura Bon, Hamada Tohru, Harayama Shigeaki, Fujii Tateo
Int J Syst Evol Microbiol. 2002 May;52(Pt 3):739-747. doi: 10.1099/00207713-52-3-739.
The phylogenetic relationships of Oceanospirillum strains were analysed by using the nucleotide sequences of 16S rRNA and gyrB genes. Results from sequence analysis demonstrated that the Oceanospirillum core group consisted of four species, Oceanospirillum linum, Oceanospirillum maris, Oceanospirillum beijerinckii and Oceanospirillum multiglobuliferum, with enough distance to separate them as different species. However, four other Oceanospirillum species occupied taxonomic positions separate from the Oceanospirillum core group: Oceanospirillum jannaschii, Oceanospirillum japonicum and Oceanospirillum kriegii in the gamma-Proteobacteria and Oceanospirillum pusillum in the alpha-Proteobacteria. Oceanospirillum jannaschii clustered with Marinobacterium georgiense, Pseudomonas iners and Pseudomonas stanieri on the basis of phylogenetic analysis of 16S rRNA and gyrB genes. The other three species did not cluster with known genera. Also, the sequence similarity values of the gyrB genes between the three subspecies of Oceanospirillum maris and those between the two subspecies of Oceanospirillum beijerinckii were above 99%. The close relationships between the subspecies of Oceanospirillum maris and of Oceanospirillum beijerinckii were further supported by similar physiological properties and high DNA-DNA hybridization values, suggesting that these subspecies should not be regarded as valid. From these results, Oceanospirillum sensu stricto should be defined to consist of Oceanospirillum linum, Oceanospirillum maris, Oceanospirillum beijerinckii and Oceanospirillum multiglobuliferum. We propose to create the following new genera: Pseudospirillum gen. nov. for Oceanospirillum japonicum as Pseudospirillum japonicum comb. nov.; Oceanobacter gen. nov. for Oceanospirillum kriegii as Oceanobacter kriegii comb. nov.; and Terasakiella gen. nov. for Oceanospirillum pusillum as Terasakiella pusilla comb. nov. The transfer is proposed of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov. Furthermore, Pseudomonas iners should be reclassified as a strain of Marinobacterium georgiense. Finally, the subspecies of Oceanospirillum maris (O. maris subsp. maris, O. maris subsp. hiroshimense and O. maris subsp. williamsae) and Oceanospirillum beijerinckii (O. beijerinckii subsp. beijerinckii and O. beijerinckii subsp. pelagicum) should be combined as Oceanospirillum maris and Oceanospirillum beijerinckii, respectively.
利用16S rRNA和gyrB基因的核苷酸序列分析了嗜盐螺菌菌株的系统发育关系。序列分析结果表明，嗜盐螺菌核心群由四个物种组成，即亚麻嗜盐螺菌、海生嗜盐螺菌、拜氏嗜盐螺菌和多球嗜盐螺菌，它们之间的距离足以将它们区分为不同的物种。然而，其他四个嗜盐螺菌物种占据了与嗜盐螺菌核心群不同的分类位置：γ-变形菌纲中的詹氏嗜盐螺菌、日本嗜盐螺菌和克里格嗜盐螺菌，以及α-变形菌纲中的微小嗜盐螺菌。基于16S rRNA和gyrB基因的系统发育分析，詹氏嗜盐螺菌与乔治海杆菌、惰性假单胞菌和斯坦尼尔假单胞菌聚类在一起。其他三个物种没有与已知属聚类。此外，海生嗜盐螺菌的三个亚种之间以及拜氏嗜盐螺菌的两个亚种之间gyrB基因的序列相似性值均高于99%。海生嗜盐螺菌和拜氏嗜盐螺菌亚种之间的密切关系进一步得到了相似生理特性和高DNA-DNA杂交值的支持，这表明这些亚种不应被视为有效种。根据这些结果，狭义嗜盐螺菌应定义为由亚麻嗜盐螺菌、海生嗜盐螺菌、拜氏嗜盐螺菌和多球嗜盐螺菌组成。我们建议创建以下新属：将日本嗜盐螺菌归为新属假螺菌属，即日本假螺菌新组合；将克里格嗜盐螺菌归为新属海洋杆菌属，即克里格海洋杆菌新组合；将微小嗜盐螺菌归为新属寺崎菌属，即微小寺崎菌新组合。建议将詹氏嗜盐螺菌和斯坦尼尔假单胞菌转移到海杆菌属，分别为詹氏海杆菌新组合和斯坦尼尔海杆菌新组合。此外，惰性假单胞菌应重新分类为乔治海杆菌的一个菌株。最后，海生嗜盐螺菌的亚种（海生嗜盐螺菌海生亚种、海生嗜盐螺菌广岛亚种和海生嗜盐螺菌威廉姆斯亚种）和拜氏嗜盐螺菌的亚种（拜氏嗜盐螺菌拜氏亚种和拜氏嗜盐螺菌远洋亚种）应分别合并为海生嗜盐螺菌和拜氏嗜盐螺菌。
