Eilat E, Zinger H, Nyska A, Mozes E
Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
J Clin Immunol. 2000 Jul;20(4):268-78. doi: 10.1023/a:1006663519132.
Two peptides based on the complementarity-determining regions (CDR) of a pathogenic murine anti-DNA antibody were employed in an attempt to prevent the spontaneous systemic lupus erythematosus (SLE)-like disease of (NZBxNZW)F1 mice. Female mice, at the age of 2 months, were injected with either the CDR1- or the CDR3-based peptides (pCDR1, pCDR3) subcutaneously or intravenously in aqueous solution for a total of 8-10 treatments. A reduction was observed in the total and pathogenic IgG2a and IgG3 anti-DNA antibody titers in the CDR-treated groups. Treatment reduced the number of mice that developed proteinuria and immune complex deposits in their kidneys. The severity of renal pathology was significantly reduced in the pCDR3 (P<0.02) and pCDR1 (P< or = 0.05) treated mice. Thus, both CDR-based peptides administered in aqueous solution were capable of preventing the SLE-like disease in (NZBxNZW)F1 mice, although the beneficial effects of pCDR3 appeared to be more pronounced than those of pCDR1 in the treated mice.
基于致病性小鼠抗DNA抗体互补决定区（CDR）的两种肽被用于尝试预防（NZBxNZW）F1小鼠的自发性系统性红斑狼疮（SLE）样疾病。2月龄雌性小鼠分别皮下或静脉注射基于CDR1或CDR3的肽（pCDR1、pCDR3）的水溶液，共进行8至10次治疗。在接受CDR治疗的组中，总抗DNA抗体以及致病性IgG2a和IgG3抗DNA抗体滴度均有所降低。治疗减少了出现蛋白尿和肾脏免疫复合物沉积的小鼠数量。在接受pCDR3（P<0.02）和pCDR1（P≤0.05）治疗的小鼠中，肾脏病理严重程度显著降低。因此，两种基于CDR的肽在水溶液中给药均能够预防（NZBxNZW）F1小鼠的SLE样疾病，尽管在接受治疗的小鼠中，pCDR3的有益效果似乎比pCDR1更显著。
