Cui X, Lee S J, Kim S Z, Kim S H, Cho K W
Department of Physiology, Medical School and Institute for Medical Sciences, Jeonbug National University, 2-20 Keum-Am-Dong-San, Jeonju 561-180, South Korea.
Eur J Pharmacol. 2000 Aug 18;402(1-2):129-37. doi: 10.1016/s0014-2999(00)00514-8.
Although pituitary adenylate cyclase activating polypeptide (PACAP) has been shown to increase cardiac force of contraction and to change the heart rate, the effect of PACAP on cyclic (c) AMP production in the atrium still has to be defined. In the present experiments, a simple protocol was developed for the evaluation of cAMP production in real-time base in the perfused beating left atria. The PACAP27-induced cAMP efflux in the atrial perfusate reflected changes in the production of cAMP in the atrial tissue. cAMP efflux was measured as an indicator of cAMP production in beating perfused rabbit atria. PACAP27 increased cAMP production in a dose- and time-dependent manner with a minor effect on atrial dynamics. These results suggest that PACAP27 has other roles besides control of force of contraction through cAMP production in the atrium.
尽管垂体腺苷酸环化酶激活多肽（PACAP）已被证明可增强心脏收缩力并改变心率，但PACAP对心房中环磷酸（c）AMP生成的影响仍有待明确。在本实验中，我们开发了一种简单的方案，用于实时评估灌注跳动的左心房中的cAMP生成。心房灌注液中PACAP27诱导的cAMP流出反映了心房组织中cAMP生成的变化。cAMP流出被用作跳动的灌注兔心房中cAMP生成的指标进行测量。PACAP27以剂量和时间依赖性方式增加cAMP生成，对心房动力学影响较小。这些结果表明，PACAP27除了通过心房中cAMP生成来控制收缩力外，还具有其他作用。
