School of Oriental Medicine & College of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea ; Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:404713. doi: 10.1155/2013/404713. Epub 2013 Oct 29.
The seeds of Lepidium apetalum Willdenow (called "Tinglizi" in China and "Jungryukza" in Korea) have been used to discharge phlegm and improve dropsy in Oriental medicine. The present study investigated the effects of ethanol extract of the seeds of Lepidium apetalum (ELA) on atrial dynamics and atrial natriuretic peptide (ANP) secretion in beating rabbit atria. ELA increased atrial stroke volume, pulse pressure, and cAMP efflux, concomitantly attenuating ANP secretion in a dose-dependent manner. ELA-induced increases in atrial stroke volume, pulse pressure, and cAMP levels and decrease in ANP secretion were not inhibited by pretreatment with staurosporine, a nonspecific protein kinase inhibitor, or diltiazem and verapamil, the L-type Ca(2+) channel blockers, respectively. Helveticoside, a well-known digitalis-like cardiac glycosidic constituent of ELA, also increased atrial dynamics, including stroke volume and pulse pressure, without changing cAMP efflux and ANP secretion, and the effects of helveticoside were not inhibited by pretreatment with staurosporine, diltiazem, and verapamil. These results suggest that the ELA-induced positive inotropic activity in beating rabbit atria might, at least partly, be due to the digitalis-like activity of helveticoside rather than an increase in cAMP efflux.
芝麻菜（在中国被称为“莙荙菜”，在韩国被称为“Jungryukza”）的种子已被用于在东方医学中排痰和治疗水肿。本研究调查了芝麻菜种子的乙醇提取物（ELA）对跳动兔心房动力学和心房利钠肽（ANP）分泌的影响。ELA 可增加心房每搏量、脉搏压和 cAMP 外排，并呈剂量依赖性地减轻 ANP 分泌。ELA 诱导的心房每搏量、脉搏压和 cAMP 水平增加以及 ANP 分泌减少，不受非特异性蛋白激酶抑制剂星形孢菌素、L 型钙 (Ca 2+ ) 通道阻滞剂地尔硫卓和维拉帕米预处理的抑制。ELA 中的一种众所周知的拟洋地黄类心脏糖苷成分——芝麻菜苷，也可增加心房动力学，包括每搏量和脉搏压，而不改变 cAMP 外排和 ANP 分泌，并且芝麻菜苷的作用不受星形孢菌素、地尔硫卓和维拉帕米预处理的抑制。这些结果表明，ELA 诱导跳动兔心房的正性肌力作用可能至少部分归因于芝麻菜苷的拟洋地黄活性，而不是 cAMP 外排的增加。
