Sresty TV
Department of Botany, Andhra University, 530 003, A.P., Visakhapatnam, India
Plant Sci. 2000 Aug 8;157(1):113-128. doi: 10.1016/s0168-9452(00)00273-9.
The zinc (Zn) and nickel (Ni) as oxidative stress factors and associated responses of 6-day-old seedlings of two pigeonpea (Cajanus cajan (L.) Millspaugh) cultivars namely LRG30 and ICPL87 were studied. Zinc and Ni exposure increased lipid peroxidation in relation to their concentration. Reduction in dry matter accumulation of roots and shoots was noticed in Zn and Ni treatments. The activities of antioxidative enzymes such as superoxide dismutase, peroxidase and glutathione reductase registered higher values and the activity of catalase and the antioxidative substances such as ascorbic acid and total glutathione contents registered lower values in all the Zn and Ni treatments when compared to their controls. The levels of catalase, peroxidase and glutathione reductase and ascorbic acid and total glutathione contents were high in cv. LRG30 than in cv. ICPL87 in response to Zn and Ni treatments. However, the activity of superoxide dismutase, the major scavenger of O(2)(&z.rad;-) radical registered higher values in cv. ICPL87. The cv. LRG30 is less sensitive to Zn and Ni treatments compared to the cv. ICPL87. Correlation coefficients between the different antioxidant parameters and metal dose level, or dry matter accumulation, were established, assessing for an induced-oxidative stress. Additional evidence was provided by comparing the sensitivity of the two cultivars.
研究了锌（Zn）和镍（Ni）作为氧化应激因素对两个木豆（Cajanus cajan (L.) Millspaugh）品种（LRG30和ICPL87）6日龄幼苗的影响及相关响应。锌和镍暴露会使其脂质过氧化作用随浓度增加。在锌和镍处理中，根和茎的干物质积累减少。与对照相比，在所有锌和镍处理中，超氧化物歧化酶、过氧化物酶和谷胱甘肽还原酶等抗氧化酶的活性值较高，而过氧化氢酶的活性以及抗坏血酸和总谷胱甘肽含量等抗氧化物质的活性值较低。在锌和镍处理下，LRG30品种中过氧化氢酶、过氧化物酶和谷胱甘肽还原酶的水平以及抗坏血酸和总谷胱甘肽含量均高于ICPL87品种。然而，超氧化物歧化酶（O(2)(&z.rad;-)自由基的主要清除剂）的活性在ICPL87品种中较高。与ICPL87品种相比，LRG30品种对锌和镍处理的敏感性较低。建立了不同抗氧化参数与金属剂量水平或干物质积累之间的相关系数，以评估诱导氧化应激。通过比较两个品种的敏感性提供了额外的证据。
