Root-knot nematodes ( spp.) are a major threat to pomegranate cultivation. Nanoparticles (NPs) present a possible substitute nematicide that lessens dependency on potentially dangerous chemical nematicides. This study assessed the efficacy of copper oxide (CuO) and iron oxide (FeO) NPs to promote pomegranate ( L. cultivar Hegazy) growth and provide protection against the root-knot nematode (). The application of the NPs as copper oxide (CuO) and iron oxide (FeO) involved both drenching and spraying using 50 mg/L on one-year-old pomegranate ( cultivar Hegazy) seedlings, nematode-infected with (). By assessing how CuO and FeO NPs affect nematode and pomegranate growth, and some biochemical traits. Treatments with NPs successfully reduced the number of pomegranate root egg masses, galls, and juvenile nematodes in soil. NP treatments exhibited increased side branching, leaf area, levels of photosynthetic pigments (chlorophyll , , and carotenoids), total antioxidants, thiol compounds [glutathione (GSH), non-protein thiols (NPTs), protein thiols (PTs)], and flavonoids. However, NP treatments reduced the accumulation of malondialdehyde (MDA) and proline, stress markers, in pomegranate plants infected with nematodes. NP treatments did not affect the production of phenolic compounds in pomegranates. These results indicate that the NP effect partially depends on the increased production of photosynthetic pigments, thiol compounds, and flavonoids. These results elucidate how nanoparticles control nematode infection. Further research in this area is necessary to determine whether NPs are the best treatment for nematode infections.