Atwood D G, Crawford B J, Braybrook G D
J Microsc. 1975 Mar;103(2):259-64. doi: 10.1111/j.1365-2818.1975.tb03901.x.
A technique for preparing heavily mucous coated marine invertebrate spermatozoa for scanning electron microscopy (SEM) is described. This technique involves washing in 1500 NF units/ml hyaluronidase in millipored sea water to remove mucus, followed by fixation in glutaraldehyde and osmium tetroxide. Following primary fixation, spermatozoa are enclosed in Nuclepore membrane bags positioned within Teflon specimen capsules allowing them to be processed and critical point dried without excessive mechanical damage or loss.
描述了一种用于制备用于扫描电子显微镜（SEM）的带有大量黏液涂层的海洋无脊椎动物精子的技术。该技术包括在经微孔过滤的海水中用1500 NF单位/毫升的透明质酸酶洗涤以去除黏液，随后用戊二醛和四氧化锇固定。初次固定后，精子被封装在置于特氟龙标本胶囊内的核孔膜袋中，以便在处理和临界点干燥过程中不会受到过度的机械损伤或损失。
