Buckland-Nicks John
Biology Department, St. Francis Xavier University, Antigonish, Nova Scotia, Canada.
Methods Mol Biol. 2014;1128:125-45. doi: 10.1007/978-1-62703-974-1_8.
Scanning electron microscopy (SEM) enables a close-up investigation of topographical features at the cellular level down to a resolution of about 3 nm. In terms of gametes this allows for examination of minute surface details and changes that occur during fertilization. Sometimes these features are hidden from view and must be carefully exposed during preparation in order to be made visible in the SEM. This chapter describes methods that can reveal surface details of gametes for observation, as well as fix them permanently, while keeping fixation artifacts to a minimum.
扫描电子显微镜（SEM）能够在细胞水平上对地形特征进行特写研究，分辨率可达约3纳米。就配子而言，这有助于检查微小的表面细节以及受精过程中发生的变化。有时这些特征会被隐藏起来，在制备过程中必须小心暴露，以便在扫描电子显微镜下可见。本章介绍了一些方法，这些方法可以揭示配子的表面细节以便观察，同时将固定假象降至最低，并对其进行永久固定。
