Otto B, Kaldenhoff R
Julius-van-Sachs-Institut für Biowissenschaften, Molekulare Pflazenphysiologie und Biophysik, Universität Würzburg, Germany.
Planta. 2000 Jul;211(2):167-72. doi: 10.1007/s004250000275.
The aquaporin NtAQP1 from Nicotiana tabacum L. is insensitive to heavy-metal ions. In addition to water, the transport of urea or glycerol is facilitated by this plasma-membrane-located water channel. Northern hybridization and whole-mount in situ hybridization revealed a high steady-state level of NtAQP1-RNA in roots, a decreased content in shoots and a low content in leaves. By immunolocalization with an antibody targeted to the N-terminus of the aquaporin, the localization of NtAQP1-protein at sites of expected high water transport rates from and to the apoplast or symplast could be demonstrated. The specific pattern of NtAQP1 distribution in petioles strongly indicates a transcellular movement of water.
烟草（Nicotiana tabacum L.）的水通道蛋白NtAQP1对重金属离子不敏感。除了水之外，这种位于质膜的水通道还促进尿素或甘油的运输。Northern杂交和整体原位杂交显示，NtAQP1-RNA在根中的稳态水平较高，在茎中的含量降低，在叶中的含量较低。通过使用针对水通道蛋白N端的抗体进行免疫定位，可以证明NtAQP1蛋白在预期的从质外体或共质体进出的高水运输速率位点的定位。NtAQP1在叶柄中的特定分布模式强烈表明水的跨细胞运动。
