Jonak C, Beisteiner D, Beyerly J, Hirt H
Institute of Microbiology and Genetics, Vienna Biocenter, University of Vienna, Dr. Bohrgasse 9, 1030 Vienna, Austria.
Plant Cell. 2000 Aug;12(8):1467-75. doi: 10.1105/tpc.12.8.1467.
Glycogen synthase kinase 3 (GSK-3) is involved in the regulation of several physiological processes, including glycogen metabolism, protein synthesis, transcription factor activity, and developmental control. Although GSK-3-like genes have been isolated from plants, no function for any of these kinases has been defined. We report here that the alfalfa wound-induced gene (WIG, for wound-induced GSK-3), lencoding a functional plant GSK-3-like kinase, is activated when the alfalfa leaves are wounded. Although WIG transcripts are hardly detectable in mature leaves, WIG mRNA accumulates rapidly after wounding. Using a peptide antibody that specifically recognizes p53(WIG), we show that p53(WIG) kinase is activated immediately after wounding. Wound-induced activation of p53(WIG) kinase is a post-translational process, because the concentrations of p53(WIG) protein do not change in intact and wounded leaves, and inhibition of transcription or translation does not block activation by wounding. However, inactivation of p53(WIG) kinase, which usually occurs within 60 min after wounding, is dependent on transcription and translation of one or more protein factors. These data suggest that the WIG kinase is involved in wound signaling in plants.
糖原合酶激酶3（GSK - 3）参与多种生理过程的调控，包括糖原代谢、蛋白质合成、转录因子活性及发育控制。尽管已从植物中分离出类GSK - 3基因，但这些激酶的功能均未明确。我们在此报告，苜蓿创伤诱导基因（WIG，即创伤诱导的GSK - 3）编码一种功能性植物类GSK - 3激酶，在苜蓿叶片受伤时被激活。尽管在成熟叶片中几乎检测不到WIG转录本，但受伤后WIG mRNA迅速积累。使用特异性识别p53（WIG）的肽抗体，我们发现p53（WIG）激酶在受伤后立即被激活。创伤诱导的p53（WIG）激酶激活是一个翻译后过程，因为完整叶片和受伤叶片中p53（WIG）蛋白的浓度没有变化，且转录或翻译的抑制并不阻断创伤诱导的激活。然而，p53（WIG）激酶的失活通常发生在受伤后60分钟内，这依赖于一种或多种蛋白质因子的转录和翻译。这些数据表明WIG激酶参与植物的创伤信号传导。
