Barattini P
Department of Physiology, Faculty of Medicine, University of Geneva, Switzerland.
Aviakosm Ekolog Med. 2000;34(3):59-61.
Duodenal ulcer is a relevant clinical condition, but information about circadian rhythms of the duodenum is scarce. While the chronobiological model of gastric ulcerogenesis has been partly demonstrated, the time dependent variations of protective and aggressive factors of the duodenal mucosa have not yet been sufficiently documented. The aim of this study is to document the duodenal mucosa circadian dynamics of glutathione (GSH), a sulfhydryl compound, a well-known intracellular protective agent. Male Wistar rats, 8-9 weeks of age, were maintained at controlled temperature and humidity and 12L:12D day cycle since birth. Duodenal mucosa samples obtained by scraping were assayed with a spectrophotometric method. The data analysis with the Cosinor method demonstrated circadian rhythmicity for GSH content in the duodenal mucosa with higher levels during the dark period.
十二指肠溃疡是一种相关的临床病症，但关于十二指肠昼夜节律的信息却很少。虽然胃溃疡发生的时间生物学模型已得到部分证实，但十二指肠黏膜保护和攻击因子的时间依赖性变化尚未得到充分记录。本研究的目的是记录谷胱甘肽（GSH），一种巯基化合物，一种著名的细胞内保护剂在十二指肠黏膜中的昼夜动态变化。8至9周龄的雄性Wistar大鼠自出生起就维持在可控的温度、湿度和12小时光照：12小时黑暗的昼夜循环环境中。通过刮取获得的十二指肠黏膜样本采用分光光度法进行检测。用余弦分析法进行数据分析表明，十二指肠黏膜中GSH含量具有昼夜节律性，在黑暗期含量较高。
