Bartha J L, Comino-Delgado R, Martinez-Del Fresno P, Ortega M J, Fernandez-Lorente J R, Cabello J M
Department of Obstetrics and Gynecology, University Hospital of Puerto Real, Cadiz, Spain.
J Reprod Med. 2000 Jul;45(7):567-71.
To evaluate lymphocyte subpopulations after a first pregnancy in women who had normal pregnancies and in those whose pregnancies terminated in spontaneous abortion.
Sixty healthy, nonpregnant women in three groups were studied: 20 with a prior abortion, 20 with a prior normal pregnancy and 20 nulligravid. Peripheral blood lymphocytes were studied using monoclonal antibodies and flow cytometry. Women were followed for one year, and if they became pregnant again, pregnancy complications were recorded.
The percentage of B lymphocytes was significantly decreased in the postpartum group (6% +/- 2.22) in comparison to nulligravid women (8.7% +/- 3.37) (P = .005). The percentage of CD4 lymphocytes was significantly higher in the postabortion group (44.7% +/- 7.81) in relation to the control group (39.85% +/- 6.01) (P = .03). A significantly higher CD4/CD8 ratio was found in the postabortion group in relation to the control group (1.65 vs. 1.24) (P = .01). Women with pregnancy complications in their next pregnancy had a lower absolute value for total lymphocytes (P = .02), T lymphocytes (P = .04), absolute CD8 lymphocytes (P = .01) and percentage of CD8 lymphocytes (P = .02) and a higher percentage of CD4 lymphocytes (P = .03) and higher CD4/CD8 ratio (P = .02) than women who had not experienced any pregnancy complications.
The percentage of B lymphocytes was lower in normal primipara in comparison to women who had never been pregnant. Women with previous spontaneous abortions had an immunologic profile expected in a rejection phenomenon; that result was more marked if they went on to experience complications in their next pregnancies.
评估正常妊娠及自然流产妇女首次妊娠后的淋巴细胞亚群。
对三组60名健康未孕妇女进行研究：20名有过流产史，20名有过正常妊娠史，20名未孕。使用单克隆抗体和流式细胞术研究外周血淋巴细胞。对妇女随访一年，若再次怀孕，记录妊娠并发症。
产后组B淋巴细胞百分比（6%±2.22）显著低于未孕妇女（8.7%±3.37）（P = 0.005）。流产后组CD4淋巴细胞百分比（44.7%±7.81）显著高于对照组（39.85%±6.01）（P = 0.03）。流产后组CD4/CD8比值显著高于对照组（1.65对1.24）（P = 0.01）。下次妊娠有并发症的妇女总淋巴细胞绝对值（P = 0.02）、T淋巴细胞（P = 0.04）、CD8淋巴细胞绝对值（P = 0.01）和CD8淋巴细胞百分比（P = 0.02）较低，CD4淋巴细胞百分比（P = 0.03）和CD4/CD8比值较高（P = 0.02），而未经历任何妊娠并发症的妇女则相反。
与从未怀孕的妇女相比，正常初产妇的B淋巴细胞百分比更低。有过自然流产史的妇女具有排斥现象中预期的免疫特征；如果她们下次妊娠出现并发症，这种结果会更明显。
