Vázquez Tsuji O, Jiménez Domínguez R, Campos Rivera T, Valencia Rojas S, Romero Cabello R, Gamez Aranda V, Martínez-Barbabosa I
Servico de Parasitología y Micología, Universidad Nacional Autónoma de México, D. F.
Rev Latinoam Microbiol. 2000 Jan-Mar;42(1):45-52.
Cyclospora cayetanensis is an Apicomplexa protozoa which was found to cause gastroenteritis in humans in 1979. This paper reviews the laboratory diagnosis of the disease. The usefulness of direct examination of fresh fecal matter with special acid-fast stains is emphasized as well as the morphometric differentiation between this organism and other similar coccidia. The paper reviews the sporulation technique of Cyclospora cayetanensis for the recognition and differentiation of artifacts and green-blue algae. Another aspect discussed is the morphology of the parasite in histological sections and with electromicroscopic examination whereby tissue morphology changes caused by the microorganism can be identified. The experience of the Service and Laboratory of Parasitology of the Instituto Nacional de Pediatría of Mexico in the laboratory diagnosis of this protozoosis is described. The paper is oriented towards the inclussion of Cyclospora cayetanensis in the diagnostic protocols for the study of diarrheas in our health Institutions.
卡耶塔环孢子球虫是一种顶复门原生动物，于1979年被发现可导致人类肠胃炎。本文综述了该疾病的实验室诊断方法。强调了使用特殊抗酸染色直接检查新鲜粪便的实用性，以及该生物体与其他类似球虫之间的形态学鉴别。本文还综述了卡耶塔环孢子球虫的孢子形成技术，用于识别和区分伪像及蓝绿藻。讨论的另一个方面是寄生虫在组织切片和电子显微镜检查中的形态，借此可识别由该微生物引起的组织形态变化。文中描述了墨西哥国家儿科研究所寄生虫学服务与实验室在该原虫病实验室诊断方面的经验。本文旨在将卡耶塔环孢子球虫纳入我国医疗机构腹泻研究的诊断方案中。
