Eberhard M L, Pieniazek N J, Arrowood M J
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Ga. 30341-3724, USA.
Arch Pathol Lab Med. 1997 Aug;121(8):792-7.
The laboratory diagnosis of newly recognized infectious agents, such as Cyclospora cayetanensis, is frequently problematic because appropriate diagnostic techniques and algorithms are not available. The methods currently available for diagnosis of Cyclospora are described and compared, including concentration procedures, examination of wet preparations, various staining techniques, and the use of molecular-based assays. Because of the autofluorescent properties of the oocysts, particular attention is drawn to the role of fluorescent microscopy in providing a rapid, inexpensive, and sensitive technique for diagnosis of Cyclospora infections in stool samples. In addition to text descriptions, photomicrographs are provided to illustrate Cyclospora oocysts in wet and stained preparations and compare them with Cryptosporidium and Isospora oocysts, the other two most common coccidian infections in man.
对新发现的感染因子，如卡耶塔环孢子虫进行实验室诊断常常存在问题，因为缺乏合适的诊断技术和方法。本文描述并比较了目前可用于诊断环孢子虫的方法，包括浓缩程序、湿片检查、各种染色技术以及基于分子的检测方法。由于卵囊的自发荧光特性，荧光显微镜在为粪便样本中环孢子虫感染的诊断提供快速、廉价且灵敏的技术方面的作用受到了特别关注。除文字描述外，还提供了显微照片，以展示湿片和染色样本中的环孢子虫卵囊，并将它们与隐孢子虫和等孢子虫的卵囊进行比较，后两者是人类另外两种最常见的球虫感染。
