Tecle T, Johansson B, Yun Z, Orvell C
Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Sweden.
Arch Virol. 2000;145(6):1199-210. doi: 10.1007/s007050070119.
Twelve strains of mumps virus, belonging to the A, C and D genotypes of the small hydrophobic (SH) protein gene, were investigated by nucleotide sequencing of the fusion protein gene. The nucleotide sequences and deduced amino acid sequences were aligned and compared with previously reported sequences of the gene. In addition an antigenic comparison between the F protein of different strains of the A, C and D genotypes was performed with ten monoclonal antibodies directed against the F protein of genotype A. Phylogenetic analysis of the coding region of the F gene showed the expected clustering of the different genotypes, as previously determined from the SH protein gene. Comparison of the 538 long amino acid sequence of the protein showed that only a small number of amino acids differed between the viral strains. The A genotype differed from B, C and D whereas the latter showed fewer consistent amino acid differences between themselves. Nine of the ten monoclonal antibodies reacted with the C and D genotypes and one failed to react with these genotypes. It is concluded that the structure and antigenicity of the F protein is well conserved both intra- and intergenotypically over long periods of time.
通过对融合蛋白基因进行核苷酸测序，研究了12株属于小疏水（SH）蛋白基因A、C和D基因型的腮腺炎病毒。将核苷酸序列和推导的氨基酸序列进行比对，并与该基因先前报道的序列进行比较。此外，用10种针对A基因型F蛋白的单克隆抗体对A、C和D基因型不同毒株的F蛋白进行了抗原性比较。F基因编码区的系统发育分析显示，不同基因型呈现出预期的聚类，这与先前根据SH蛋白基因确定的结果一致。对该蛋白538个氨基酸的长序列进行比较表明，病毒毒株之间只有少数氨基酸存在差异。A基因型与B、C和D基因型不同，而后三者之间一致的氨基酸差异较少。10种单克隆抗体中有9种与C和D基因型发生反应，1种未与这些基因型发生反应。结论是，F蛋白的结构和抗原性在基因型内和基因型间长期都得到了很好的保守。
