Chambers Phil, Rima Bert K, Duprex W Paul
Centre for Infection and Immunity, School of Medicine, Dentistry and Biomedical Sciences, Medical Biology Centre, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
J Gen Virol. 2009 Dec;90(Pt 12):2973-2981. doi: 10.1099/vir.0.013946-0. Epub 2009 Aug 5.
The Jeryl Lynn (JL) vaccine against mumps virus (MuV) contains two components, MuV(JL5) and MuV(JL2), which differ by over 400 nt. Due to the occurrence of bias in the direction of mutation, these differences and those found in nucleotide sequences of different isolates of the minor component in the vaccine (MuV(JL2)) might be due to the effect of ADAR-like deaminases on MuV grown in tissue-cultured cells. A molecular clone of MuV(JL2) (pMuV(JL2)) and MuV(JL2)-specific helper plasmids were constructed in order to investigate molecular interactions between MuV(JL5) and MuV(JL2), to augment the existing molecular clone of MuV(JL5) (pMuV(JL5)) and MuV(JL5)-specific helper plasmids. Genome and mRNA termini of MuV(JL2) were characterized, and an unusual oligo-G insertion transcriptional editing event was detected near the F mRNA polyadenylation site of MuV(JL2), but not of MuV(JL5). Genes encoding glycoproteins of rMuV(JL2) and rMuV(JL5) have been exchanged to characterize the oligo-G insertion, which associated with the specific sequence of the F gene of MuV(JL2) and not with any other genes or the RNA-dependent RNA polymerase of strain MuV(JL2). The results indicate that a single G-to-A sequence change obliterates the co-transcriptional editing of the F mRNA and that this oligo-G insertion does not affect the growth of the virus.
针对腮腺炎病毒（MuV）的杰里尔·林恩（JL）疫苗包含两个成分，即MuV（JL5）和MuV（JL2），它们相差超过400个核苷酸。由于突变方向存在偏差，这些差异以及在疫苗中次要成分（MuV（JL2））不同分离株的核苷酸序列中发现的差异，可能是由于类ADAR脱氨酶对在组织培养细胞中生长的MuV的影响。构建了MuV（JL2）的分子克隆（pMuV（JL2））和MuV（JL2）特异性辅助质粒，以研究MuV（JL5）和MuV（JL2）之间的分子相互作用，增强现有的MuV（JL5）分子克隆（pMuV（JL5））和MuV（JL5）特异性辅助质粒。对MuV（JL2）的基因组和mRNA末端进行了表征，在MuV（JL2）的F mRNA聚腺苷酸化位点附近检测到一个不寻常的寡聚G插入转录编辑事件，但在MuV（JL5）中未检测到。已交换了编码rMuV（JL2）和rMuV（JL5）糖蛋白的基因，以表征寡聚G插入，该插入与MuV（JL2）的F基因的特定序列相关，而与MuV（JL2）株的任何其他基因或RNA依赖性RNA聚合酶无关。结果表明，单个G到A的序列变化消除了F mRNA的共转录编辑，并且这种寡聚G插入不影响病毒的生长。
