Blakeney M D, Moulaei T, DiChristina T J
School of Biology, Georgia Institute of Technology, Atlanta 30332-0230, USA.
Microbiol Res. 2000 Jul;155(2):87-94. doi: 10.1016/S0944-5013(00)80042-7.
Shewanella putrefaciens was grown on a series of ten alternate compounds as sole terminal electron acceptor. Each cell type was analyzed for Fe(III) reduction activity, absorbance maxima in reduced-minus-oxidized difference spectra and heme-containing protein content. High-rate Fe(III) reduction activity, pronounced difference maxima at 521 and 551 nm and a predominant 29.3 kDa heme-containing protein expressed by cells grown on Fe(III), Mn(IV), U(VI), SO3(2-) and S2O3(2-), but not by cells grown on O2, NO3, NO2-, TMAO or fumarate. These results suggest that microbial Fe(III) reduction activity is enhanced by anaerobic growth on metals and sulfur compounds, yet is limited under all other terminal electron-accepting conditions.
腐败希瓦氏菌在一系列十种交替化合物作为唯一末端电子受体的条件下生长。对每种细胞类型进行了铁（III）还原活性、还原态减去氧化态差光谱中的吸光度最大值以及含血红素蛋白含量的分析。在以铁（III）、锰（IV）、铀（VI）、亚硫酸根（SO3(2-)）和硫代硫酸根（S2O3(2-)）为电子受体生长的细胞中表现出高比率的铁（III）还原活性、在521和551纳米处有明显的差异最大值以及一种主要的29.3 kDa含血红素蛋白，但在以氧气、硝酸根、亚硝酸根、三甲胺氧化物或富马酸为电子受体生长的细胞中则没有。这些结果表明，微生物的铁（III）还原活性通过在金属和硫化合物上进行厌氧生长而增强，但在所有其他末端电子接受条件下受到限制。
