Pratuangdejkul J, Dharmsthiti S
Center for Biotechnology, Institute for Research and Development in Science and Technology, Mahidol University, Salaya, Nakornpathom, Thailand.
Microbiol Res. 2000 Jul;155(2):95-100. doi: 10.1016/S0944-5013(00)80043-9.
A lipase-producing bacterium, Acinetobacter calcoacetius LP009, was isolated from raw milk. The optimum conditions for growth and lipase production by A. calcoaceticus LP009 were 15 degrees C with shaking at 200 rpm in LB supplemented with 1.0% (v/v) Tween 80. The crude lipase was purified to homogeneous state by ultrafiltration and gel filtration chromatography on Sephadex G-100. Its molecular weight determined by SDS-PAGE was 23 kDa and it exhibited maximum activity at pH 7.0 and 50 degrees C. It was stable over the pH range of 4.0 to 8.0 and at temperatures lower than 45 degrees C. It was a metalloenzyme that is positionally non-specific and had the ability to improve fat hydrolysis in soybean meal and in premixed animals feed.
从生牛奶中分离出一株产脂肪酶的细菌——醋酸钙不动杆菌LP009。醋酸钙不动杆菌LP009生长和产脂肪酶的最佳条件是在补充有1.0%（v/v）吐温80的LB培养基中，于15℃、200 rpm振荡培养。通过超滤和Sephadex G - 100凝胶过滤色谱将粗脂肪酶纯化至均一状态。经SDS - PAGE测定其分子量为23 kDa，在pH 7.0和50℃时表现出最大活性。在pH 4.0至8.0范围内以及低于45℃的温度下它都很稳定。它是一种金属酶，具有位置非特异性，能够提高豆粕和预混动物饲料中的脂肪水解率。
