Ogilvie A, Lämmerman M, Wiebauer K, Kersten W
Biochim Biophys Acta. 1975 Jun 16;395(2):136-45.
The mode of action of a synthetic cytostatic quinone was studied in Escherichia coli. 1. At concentrations of 1.5-6 mug/ml, 5,8-dioxo-6-amino-7-chloroquinoline rapidly inhibits growth and protein synthesis in E. coli. The synthesis of RNA is immediately affected in E. coli rel+ whereas in E. coli rel- the accumulation of RNA can proceed on addition of the quinone. This indicates that the inhibition of RNA synthesis in the stringent strain is a consequence of the regulatory phenomenon governed by the rel gene. 2. Chloramphenicol, known to abolish the stringent control mechanism, added simultaneously with the quinone allows the accumulation of RNA to proceed in the stringent strain. 3. Guanosine tetraphosphate accumulates in quinone-treated E. coli rel+ but not in the relaxed mutant strain. 4. Addition of amino acids reverses all inhibitory effects observed in quinone treated stringent and relaxed cells. 5. It is concluded that the bacteriostatic effect of 5,8-dioxo-6-amino-7-chloroquinoline on E. coli is caused by an apparent intracellular amino acid starvation.
在大肠杆菌中研究了一种合成的细胞生长抑制剂醌的作用方式。1. 在浓度为1.5 - 6微克/毫升时，5,8 - 二氧代 - 6 - 氨基 - 7 - 氯喹啉能迅速抑制大肠杆菌的生长和蛋白质合成。在rel +型大肠杆菌中，RNA的合成立即受到影响，而在rel -型大肠杆菌中，添加醌后RNA的积累仍可继续进行。这表明在严谨型菌株中RNA合成的抑制是由rel基因控制的调节现象的结果。2. 已知能消除严谨控制机制的氯霉素与醌同时添加时，可使严谨型菌株中RNA的积累继续进行。3. 四磷酸鸟苷在经醌处理的rel +型大肠杆菌中积累，但在松弛型突变菌株中不积累。4. 添加氨基酸可逆转在经醌处理的严谨型和松弛型细胞中观察到的所有抑制作用。5. 得出结论：5,8 - 二氧代 - 6 - 氨基 - 7 - 氯喹啉对大肠杆菌的抑菌作用是由明显的细胞内氨基酸饥饿引起的。
