Muto A, Kimura A, Osawa S
Mol Gen Genet. 1975 Sep 8;139(4):321-7. doi: 10.1007/BF00267972.
Effects of neomycin, spectinomycin, tetracycline and chloramphenicol on the stringent control RNA synthesis and on ppGpp synthesis in the rel+-cells of Escherichia coli having a temperature-sensitive valyl-tRNA synthetase were examined. Without antibiotics, ppGpp began to accumulate and both RNA and protein syntheses were inhibited by transferring the exponentially growing cells from 30 degrees C (permissive temp.) to 40 degrees C (non-permissive temp.). Tetracycline or chloramphenicol, when added after the temperature shift, caused a resumption of RNA synthesis and decay of the accumulated ppGpp, while neomycin or spectinomycin had little effect both on RNA synthesis and the level of ppGpp. When the cells were treated with these antibiotics at permissive temperature, the shift of the temperature to 40 degrees C caused neither inhibition of RNA synthesis nor an accumulation of ppGpp. When neomycin or spectinomycin was added at the beginning of the temperature shift, RNA synthesis continued with an accumulation of ppGpp. Tetracycline or chloramphenicol had no such effect under the same conditions; RNA synthesis continued without an accumulation of ppGpp.
研究了新霉素、壮观霉素、四环素和氯霉素对具有温度敏感型缬氨酰 - tRNA合成酶的大肠杆菌rel⁺细胞中严格控制RNA合成及ppGpp合成的影响。在无抗生素情况下，当将指数生长期的细胞从30℃（允许温度）转移至40℃（非允许温度）时，ppGpp开始积累，RNA和蛋白质合成均受到抑制。温度转变后添加四环素或氯霉素，会使RNA合成恢复且积累的ppGpp衰减，而新霉素或壮观霉素对RNA合成及ppGpp水平几乎没有影响。当细胞在允许温度下用这些抗生素处理时，温度转变至40℃既不会抑制RNA合成，也不会导致ppGpp积累。当在温度转变开始时添加新霉素或壮观霉素，RNA合成会继续且伴有ppGpp积累。在相同条件下，四环素或氯霉素没有这种作用；RNA合成继续但无ppGpp积累。
