Kaplan S, Atherly A G, Barrett A
Proc Natl Acad Sci U S A. 1973 Mar;70(3):689-92. doi: 10.1073/pnas.70.3.689.
It has been possible to demonstrate the complete absence of either charged tRNA(Glu) or charged tRNA(Val) at 42 degrees by the use of two stringent strains of E. coli, one temperature-sensitive for glutamyl-tRNA synthetase and the other temperature-sensitive for valyl-tRNA synthetase. In both strains, stable RNA synthesis ceases, and guanosine tetraphosphate accumulates upon incubation at the nonpermissive temperature. Unique among a series of antibiotics tested, only tetracycline was able to stimulate stable RNA synthesis and to cause disappearance of the guanosine nucleotide. In this regard tetracycline and the "relaxed" gene product appear to be analogous.
通过使用两株严格的大肠杆菌菌株，已经有可能证明在42摄氏度时，带电荷的tRNA（Glu）或带电荷的tRNA（Val）完全不存在。其中一株对谷氨酰胺-tRNA合成酶温度敏感，另一株对缬氨酸-tRNA合成酶温度敏感。在这两种菌株中，稳定RNA合成停止，并且在非允许温度下孵育时四磷酸鸟苷会积累。在一系列测试的抗生素中，只有四环素能够刺激稳定RNA合成并导致鸟苷核苷酸消失。在这方面，四环素和“松弛”基因产物似乎是类似的。
