Rabinovich P M, Kliachko E V, Shakulov R S
Biokhimiia. 1975 Jan-Feb;40(1):187-91.
The dissociation of ribosomes from two isogenic pairs of Escherichia coli strains was studied during exponential growth, under amino acid starvation and subsequent chloramphenicol treatment. There were no significant differences in Mg2+-dependent dissociation of ribosomes from exponentially growing rel+ and rel- minus strains. The differences in dissociation of the ribosomes from rel+ and rel- minus cells were observed only upon amino acid starvation of these cultures. The dissociation of ribosomes from starved rel+ cells was more complete. After chloramphenicol treatment isolated ribosomes were more resistant to dissociation into subunits. Alterations of dissociation of the ribosomes in vitro correlated both with the amount of polysomes and the level of RNA synthesis in cells. It is proposed that rRNA synthesis in bacteria depends on the ratio of programmed and deprogrammed ribosomes.
在指数生长期、氨基酸饥饿及随后氯霉素处理期间，研究了两对等基因大肠杆菌菌株核糖体的解离情况。在指数生长期的rel⁺和rel⁻菌株中，核糖体的Mg²⁺依赖性解离没有显著差异。只有在这些培养物氨基酸饥饿时，才观察到rel⁺和rel⁻细胞核糖体解离的差异。饥饿rel⁺细胞的核糖体解离更完全。氯霉素处理后，分离的核糖体对解离成亚基更具抗性。体外核糖体解离的改变与多核糖体的数量和细胞中RNA合成水平均相关。有人提出细菌中的rRNA合成取决于程序化和非程序化核糖体的比例。
