Wada A, Igarashi K, Yoshimura S, Aimoto S, Ishihama A
Department of Physics, Faculty of Science, Kyoto University, Japan.
Biochem Biophys Res Commun. 1995 Sep 14;214(2):410-7. doi: 10.1006/bbrc.1995.2302.
Ribosome modulation factor (RMF) is an Escherichia coli protein associated with 100S ribosome dimers, which are formed at stationary growth phase or in slowly growing cells. RMF either purified from stationary-phase ribosomes or synthesized by a chemical method was examined for its functions. By adding either natural or synthetic RMF to 70S ribosomes prepared from both exponentially growing and stationary phase cells, 100S ribosome dimers were generated in a concentration-dependent manner. Protein synthesis in vitro was inhibited concomittantly with the formation of 100S ribosomes. The binding of aminoacyl-tRNA to ribosomes was inhibited in parallel. Taken together we propose that RMF is a stationary phase-specific inhibitor of ribosome functions and 100S dimers are stored forms of ribosomes.
核糖体调控因子（RMF）是一种与100S核糖体二聚体相关的大肠杆菌蛋白，100S核糖体二聚体在稳定生长期或生长缓慢的细胞中形成。对从稳定期核糖体中纯化或通过化学方法合成的RMF的功能进行了研究。通过将天然或合成的RMF添加到由指数生长期细胞和稳定期细胞制备的70S核糖体中，以浓度依赖的方式生成了100S核糖体二聚体。体外蛋白质合成与100S核糖体的形成同时受到抑制。氨酰-tRNA与核糖体的结合也受到平行抑制。综上所述，我们提出RMF是核糖体功能的稳定期特异性抑制剂，100S二聚体是核糖体的储存形式。
