Heggebø Ragna, Press Charles McL, Gunnes Gjermund, Inge Lie Kai, Tranulis Michael A, Ulvund Martha, Groschup Martin H, Landsverk Thor
Department of Morphology, Genetics and Aquatic Biology1 and Department of Biochemistry, Physiology and Nutrition2, Norwegian School of Veterinary Science, PO Box 8146 Dep., N-0033, Oslo, Norway.
Department of Sheep and Goat Research, Norwegian School of Veterinary Science, Kyrkjevegen 332/334, 4300 Sandnes, Norway3.
J Gen Virol. 2000 Sep;81(Pt 9):2327-2337. doi: 10.1099/0022-1317-81-9-2327.
A sensitive immunohistochemical procedure was used to investigate the presence of prion protein (PrP) in the ileal Peyer's patch of PrP-genotyped lambs, including scrapie-free lambs and lambs naturally and experimentally exposed to the scrapie agent. The tyramide signal amplification system was used to enhance the sensitivity of conventional immunohistochemical procedures to show that PrP was widely distributed in the enteric nervous plexus supplying the gut wall. In scrapie-free lambs, PrP was also detected in scattered cells in the lamina propria and in the dome and interfollicular areas of the Peyer's patch. In the follicles, staining for PrP was mainly confined to the capsule and cells associated with vascular structures in the light central zone. In lambs naturally exposed to the scrapie agent, staining was prominent in the dome and neck region of the follicles and was also found to be associated with the follicle-associated epithelium. Similar observations were made in lambs that had received a single oral dose of scrapie-infected brain material from sheep with a homologous and heterologous PrP genotype 1 and 5 weeks previously. These studies show that the ileal Peyer's patch in young sheep may be an important site of uptake of the scrapie agent and that the biology of this major gut-associated lymphoid tissue may influence the susceptibility to oral infection in sheep. Furthermore, these studies suggest that homology or heterology between PrP genotypes or the presence of PrP genotypes seldom associated with disease does not impede uptake of PrP.
采用一种灵敏的免疫组织化学方法，研究经朊病毒蛋白（PrP）基因分型的羔羊回肠派伊尔结中PrP的存在情况，这些羔羊包括未感染羊瘙痒病的羔羊以及自然和实验性接触羊瘙痒病病原体的羔羊。使用酪胺信号放大系统增强传统免疫组织化学方法的敏感性，结果显示PrP广泛分布于供应肠壁的肠神经丛中。在未感染羊瘙痒病的羔羊中，在固有层以及派伊尔结的圆顶区和滤泡间区的散在细胞中也检测到了PrP。在滤泡中，PrP染色主要局限于囊以及光中心区与血管结构相关的细胞。在自然接触羊瘙痒病病原体的羔羊中，滤泡的圆顶区和颈部区域染色明显，并且还发现与滤泡相关上皮有关。在1周和5周前经口单次给予来自具有同源和异源PrP基因型1的羊的羊瘙痒病感染脑材料的羔羊中也有类似观察结果。这些研究表明，幼羊的回肠派伊尔结可能是羊瘙痒病病原体摄取的重要部位，并且这种主要的肠道相关淋巴组织的生物学特性可能影响绵羊经口感染的易感性。此外，这些研究表明，PrP基因型之间的同源性或异源性，或很少与疾病相关的PrP基因型的存在并不妨碍PrP的摄取。
