Heggebø R, Press C McL, Gunnes G, Ulvund M J, Tranulis M A, Lsverk T
Department of Sheep and Goat Research, Norwegian School of Veterinary Science, Kyrkjevegen 332/334, N-4325, Sandnes, Norway
J Comp Pathol. 2003 Feb-Apr;128(2-3):172-81. doi: 10.1053/jcpa.2002.0625.
Histoblotting and immunohistochemistry were used to detect disease-associated prion protein (PrP(Sc)) in lymphoid tissues of lambs of known PrP genotype infected with the scrapie agent by stomach tube at the age of 2 months. The ileal and jejunal Peyer's patches and retropharyngeal and distal jejunal lymph nodes were studied 1 week, 5 weeks, 5 months and 11 months after inoculation. Other lymphoid tissues examined included superficial cervical lymph node, tonsil and spleen. PrP(Sc) was not detected in any tissue of any lamb at 1 week post-inoculation. At 5 weeks, PrP(Sc) was detected in tissues of lambs of susceptible PrP genotypes (AV(136)QQ(171) and VV(136)QQ(171)), but not lambs of other PrP genotypes (AA(136)QQ(171), AA(136)QR(171) and AV(136)QR(171)). PrP(Sc) was present in the germinal centres of tonsils, distal jejunal and retropharyngeal lymph nodes, and spleen. In the nodules of ileal and jejunal Peyer's patches, only occasional solitary cells showed the presence of PrP(Sc). At 5 months post-inoculation, increased accumulations of PrP(Sc) were detected in ileal and jejunal Peyer's patches, as well as in the retropharyngeal and distal jejunal lymph nodes of a single lamb inoculated with the agent from a sheep of the same susceptible PrP genotype. Eleven months after exposure to the scrapie agent, PrP(Sc) was detected in all lymphoid tissues examined from sheep of susceptible PrP genotypes. These studies show that PrP(Sc) was detectable in lymphoid tissues 5 weeks after exposure to the scrapie agent by stomach tube in lambs as young as 3 months of age and indicate that the PrP genotype is a significant factor for the rapid uptake and spread of the agent through lymphoid tissues.
采用组织印迹法和免疫组织化学法，检测2月龄经胃管接种羊瘙痒病病原体的已知朊蛋白基因型羔羊淋巴组织中的疾病相关朊蛋白（PrP(Sc)）。在接种后1周、5周、5个月和11个月，对回肠和空肠派伊尔结以及咽后和空肠远端淋巴结进行了研究。检查其他淋巴组织包括颈浅淋巴结、扁桃体和脾脏。接种后1周，在任何羔羊的任何组织中均未检测到PrP(Sc)。5周时，在易感朊蛋白基因型（AV(136)QQ(171)和VV(136)QQ(171)）的羔羊组织中检测到PrP(Sc)，但在其他朊蛋白基因型（AA(136)QQ(171)、AA(136)QR(171)和AV(136)QR(171)）的羔羊中未检测到。PrP(Sc)存在于扁桃体、空肠远端和咽后淋巴结以及脾脏的生发中心。在回肠和空肠派伊尔结的小结中，仅偶尔有单个细胞显示存在PrP(Sc)。接种后5个月，在一只接种了来自相同易感朊蛋白基因型绵羊病原体的羔羊的回肠和空肠派伊尔结以及咽后和空肠远端淋巴结中，检测到PrP(Sc)的积累增加。暴露于羊瘙痒病病原体11个月后，在易感朊蛋白基因型绵羊的所有检查淋巴组织中均检测到PrP(Sc)。这些研究表明，在3月龄幼羔经胃管接触羊瘙痒病病原体5周后，其淋巴组织中可检测到PrP(Sc)，这表明朊蛋白基因型是病原体通过淋巴组织快速摄取和传播的重要因素。
