Weiss D J, Evanson O A, Green B T, Brown D R
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
Am J Vet Res. 2000 Aug;61(8):858-61. doi: 10.2460/ajvr.2000.61.858.
To study the in vitro effects of cecal contents incubated with corn starch on colonic permeability in horses.
4 healthy adult ponies.
Mucosal specimens were obtained from the right ventral colon and mounted in Ussing chambers. Changes in short circuit current, conductance, and large-molecule permeability in response to addition of cecal contents and cecal contents incubated with corn starch were evaluated for 120 minutes.
Incubation of cecal contents with corn starch for 8 hours resulted in a decrease in cecal content pH and an increase in lactic acid concentration. These changes were similar to those reported in vivo for ponies given corn starch. Exposure of colonic mucosa to cecal contents incubated with corn starch resulted in an increase in tissue conductance and permeability of technetium Tc 99m pentetate, compared with mucosa exposed to cecal contents alone.
In vitro exposure of colonic mucosa to cecal contents incubated with starch resulted in increased paracellular permeability. Fermentation of excessive amounts of carbohydrate in the intestinal lumen of horses may directly induce increased intestinal permeability associated with carbohydrate-induced laminitis.
研究与玉米淀粉一起孵育的盲肠内容物对马结肠通透性的体外影响。
4匹健康成年小马。
从右腹侧结肠获取黏膜标本并安装在尤斯灌流小室中。在120分钟内评估添加盲肠内容物以及与玉米淀粉一起孵育的盲肠内容物后短路电流、电导和大分子通透性的变化。
盲肠内容物与玉米淀粉孵育8小时导致盲肠内容物pH值降低，乳酸浓度升高。这些变化与体内给予玉米淀粉的小马所报道的变化相似。与仅暴露于盲肠内容物的黏膜相比，结肠黏膜暴露于与玉米淀粉一起孵育的盲肠内容物会导致组织电导和锝Tc 99m喷替酸盐的通透性增加。
结肠黏膜在体外暴露于与淀粉一起孵育的盲肠内容物会导致细胞旁通透性增加。马肠腔内过量碳水化合物的发酵可能直接导致与碳水化合物诱导的蹄叶炎相关的肠通透性增加。
